DEVELOPMENT OF NON-TIDAL ADAPTIVE RICE VARIETIES: MOLECULAR MARKER ASSISTED SELECTION OF BC2F1 PROGENIES

Authors

  • Nabilah Amiros Universitas Sriwijaya
  • Rujito Agus Suwignyo Universitas Sriwijaya
  • Mery Hasmeda Universitas Sriwijaya
  • Entis Sutisna Halimi Universitas Sriwijaya
  • Fikri Adriansyah Universitas Sriwijaya

Article Metrics

This article Abstract has been read: 44 times
PDF Downloads: 23 times
Total Galley Downloads: 23 times
Total Views: 67 times

DOI:

https://doi.org/10.24233/biov.8.2.2022.332

Keywords:

dual tolerance , marker assisted backcrossing , Sub1 gene

Abstract

Submergence stress and drought stress are main abiotic constraints encountered in South Sumatra swampland rice cultivation area. The development of new abiotic stress tolerant varieties through the introgresion of tolerance genes, such as the Sub1 gene (submergence tolerance) by using Marker-assisted Backcrossing (MABC method) is one of an ideal solution to obtain an adaptive rice variety for those conditions. The study was carried out at Faculty of Agriculture, Sriwijaya University, Indonesia. In this study, BC2F1 used as the plant materials derived from backcross performed in BC1F1 and the recipient parent, Inpago 5. Previously, BC1F1 obtained from backcrossed progenies from Inpara 8 (inherited Sub1A gene) and Inpago 5 as recipient parent, a drought tolerant variety. The main objective of this study was to analyze the Sub1 introgressed plants in backcrossed progeny BC2F1 that closest similarity to recipient parent. The result showed that Sub1 introgression was confirmed by a tightly linked Sub1 gene marker, SUB1C173 marker. Out of 47 plants, 20 plants were selected based on foreground selection. Those plants were further analyzed on background selection by using 13 SSR markers. Based on the foreground and background selection, two plants, viz, plant no. 41 and 44, were selected and will be used for further study.

Last Year PDF Downloads

Download data is not yet available.

References

BPS (Central Statistic Agency), “Catalog : 1101001,” Stat. Indones. 2020, vol. 1101001, p. 790, 2020, [Online]. Available: https://www.bps.go.id/publication/2020/04/29/e9011b3155d45d70823c141f/statistik-indonesia-2020.html.

BPS (Central Statistic Agency), South Sumatra in Figure 2015. Central Statistics Agency of South Sumatra Province, Palembang, 2014.

Muhakka, R. A. Suwignyo, D. Budianta, and Yakup, “Vegetation Analysis of Non-tidal Swampland in South Sumatra, Indonesia and Its Carrying Capacity for Pampangan Buffalo Pasture,” Biodiversitas, vol. 20, no. 4, pp. 1077–1086, 2019, doi: 10.13057/biodiv/d200420.

M. Chozin, Sumardi, S. Sudjamiko, and M. F. Barchia, “Genetic variability and traits association analyses on F2 generations for determination of selection criteria in Indonesian inland swamp rice breeding,” Aust. J. Crop Sci., vol. 11, no. 5, pp. 535–541, 2017, doi: 10.21475/ajcs.17.11.05.p317.

Y. Oladosu et al., “Submergence tolerance in rice: Review of mechanism, breeding and, future prospects,” Sustain., vol. 12, no. 4, pp. 1–16, 2020, doi: 10.3390/su12041632.

R. H. Wening, B. S. Purwoko, W. B. Suwarno, I. A. Rumanti, and N. Khumaida, “Submergence and drought stresses in rice over genotype by environment interaction,” Sabrao J. Breed. Genet., vol. 52, no. 4, pp. 435–445, 2020.

K. Xu and D. J. Mackill, “A major locus for submergence tolerance mapped on rice chromosome 9,” Mol. Breed., vol. 2, no. 3, pp. 219–224, 1996, doi: 10.1007/BF00564199.

K. Xu et al., “Sub1A is An Ethylene Response Factor-Like Gene that Confers Submergence Tolerance to Rice,” Nature, vol. 442, pp. 705–707, 2006.

I. A. Rumanti et al., “Development of High-Yielding Rice Varieties Suitable for Swampy Lands in Indonesia,” Plant Breed. Biotechnol., vol. 4, no. 4, pp. 413–425, 2016, doi: 10.9787/pbb.2016.4.4.413.

T. Fukao, E. Yeung, and J. Bailey-serres, “The Submergence Tolerance Regulator SUB1A Mediates Crosstalk between Submergence and Drought Tolerance in Rice,” vol. 23, no. January, pp. 412–427, 2011, doi: 10.1105/tpc.110.080325.

A. Singh, E. M. Septiningsih, H. S. Balyan, N. K. Singh, and V. Rai, “Genetics,Pphysiological Mechanisms and breeding of flood-tolerant rice (Oryza sativa L.),” Plant Cell Physiol., vol. 58, no. 2, pp. 185–197, 2017, doi: 10.1093/pcp/pcw206.

F. Adriansyah, M. Hasmeda, R. A. Suwignyo, E. S. Halimi, and U. Sarimana, “Improvement of the submergence tolerance of local South Sumatran rice through the introgression of the Sub1 gene by using marker-assisted selection,” Sabrao J. Breed. Genet., vol. 53, no. 4, pp. 575–591, 2021, doi: https://doi.org/10.54910/sabrao2021.53.4.3.

K. M. Iftekharuddaula, H. U. Ahmed, S. Ghosal, Z. R. Moni, A. Amin, and M. S. Ali, “Development of New Submergence Tolerant Rice Variety for Bangladesh Using Marker-Assisted Backcrossing,” Rice Sci., vol. 22, no. 1, pp. 16–26, 2015, doi: 10.1016/j.rsci.2015.05.003.

R. A. Suwignyo, I. Irmawati, F. Hose, and S. L. Aulia, “Development of Rice Varieties Adaptive to Nontidal Swampland using MABC: Growth Characteristics of Parent Plant and F1 Result,” in IOP Conference Series: Earth and Environmental Science, 2021, vol. 741, no. 1, pp. 4–9, doi: 10.1088/1755-1315/741/1/012022.

R. D. Ningsih, M. Yasin, and A. Noor, “Rice productivity on tidal swampland in the agricultural assisitance area program in Barito Kuala Regency South Kalimantan,” IOP Conf. Ser. Earth Environ. Sci., vol. 484, no. 1, 2020, doi: 10.1088/1755-1315/484/1/012123.

S. L. Aulia. 2021. Pembentukan Populasi BC1F1 Seleksi MABC (Marker Assisted Backcrossing) dan Uji Toleransi Cekaman Terendam BC1F1 dari Hasil Persilangan Varietas Inpago 5 dan Inpara 8 (Magister thesis). Pascasarjana Universitas Sriwijaya.

E. M. Septiningsih et al., “Development of submergence-tolerant rice cultivars: The Sub1 locus and beyond,” Ann. Bot., vol. 103, no. 2, pp. 151–160, 2009, doi: 10.1093/aob/mcn206.

E. M. Septiningsih, B. C. Y. Collard, S. Heuer, J. Bailey-serres, A. Ismail, and D. J. Mackill, “Applying Genomics Tools for Breeding Submergence Tolerance in Rice,” in Translational Genomics for Crop Breeding , Volume II : Abiotic Stress , Yield and Quality, vol. II, 2013, pp. 9–30.

W. C. Mojulat et al., “Analysis of simple sequence repeat markers linked to submergence tolerance on newly developed rice lines derived from MR263 × swarna-Sub1,” Sains Malaysiana, vol. 46, no. 4, pp. 521–528, 2017, doi: 10.17576/jsm-2017-4604-02.

F. Yeh, T. Boyle, Y. Ye, and J. Xiyan, POPGENE version 1.31 Quick User Guide. C. f. I. F. R. University of Alberta and Tim Boyle, ed., 1999.

R. Van Berloo, “GGT 2.0: Versatile software for visualization and analysis of genetic data,” J. Hered., vol. 99, no. 2, pp. 232–236, 2008, doi: 10.1093/jhered/esm109.

C. . Neeraja et al., “A Marker-Assisted Backcross Approach for Developing Submergence-Tolerant Rice Cultivars,” Theor Appl Genet, vol. 115, pp. 767–776, 2007, doi: 10.1007/s00122-007-0607-0.

Published

25-12-2022

How to Cite
Write scientific names with Italic fonts:

Amiros, N., Suwignyo, R. A., Hasmeda, M., Halimi, E. S., & Adriansyah, F. (2022). DEVELOPMENT OF NON-TIDAL ADAPTIVE RICE VARIETIES: MOLECULAR MARKER ASSISTED SELECTION OF BC2F1 PROGENIES. BIOVALENTIA: Biological Research Journal, 8(2), 178–184. https://doi.org/10.24233/biov.8.2.2022.332

Issue

Section

Vol 8, No 2 (2022): Nov 2022

Most read articles by the same author(s)