THE EFFECT OF CULTURE MEDIA TYPE AND PLANT GROWTH REGULATORS ON CALLUS INDUCTION OF OIL PALM (Elaeis guineensis Jacq) PISIFERA TYPE

Authors

  • Gogoh Sulaksono Department of Agronomy, Faculty of Agriculture, University of Sriwijaya, Jl. Raya Palembang-Prabumulih Km. 32 Inderalaya, Ogan Ilir 30662, Palembang, Indonesia
  • Mery Hasmeda Department of Agronomy, Faculty of Agriculture, University of Sriwijaya, Jl. Raya Palembang-Prabumulih Km. 32 Inderalaya, Ogan Ilir 30662, Palembang, Indonesia
  • Laila Hanum Department of Biology, Faculty of Mathematics & Natural Sciences, Sriwijaya University. Jalan Raya Palembang-Prabumulih km 32, Indralaya, Indonesia
  • Fahmi Wendra Department of Research & Development, PT Sampoerna Agro Tbk, Jl. Basuki Rahmat No. 788 Palembang 30127, Indonesia; Tel +62-711 813388 Fax +62-711 811585
  • Baitha Santika Department of Research & Development, PT Sampoerna Agro Tbk, Jl. Basuki Rahmat No. 788 Palembang 30127, Indonesia; Tel +62-711 813388 Fax +62-711 811585
  • Dwi Asmono Department of Research & Development, PT Sampoerna Agro Tbk, Jl. Basuki Rahmat No. 788 Palembang 30127, Indonesia; Tel +62-711 813388 Fax +62-711 811585

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DOI:

https://doi.org/10.24233/biov.7.2.2021.224

Keywords:

tissue culture, pisifera type, callus induction, culture media, plant growth regulators

Abstract

Oil palm has a high economic value since it is one of the highest vegetable oil-producing plants compared to other oil-producing plants. The provision of good planting material for oil palm plantations is one of the determining factors to increase productivity. The parent Pisifera type of oil palm is important to develop the high-yielding oil palm Tenera varieties. The provision of oil palm material could be done through generative system with seeds and also could be done through vegetative approaches using tissue culture techniques. It aims to get plants that are genetically the same as their parents (true to type). The purpose of this study was to see the effect of different type of tissue culture media and plant growth regulators (PGR’s) on the callus induction stage of Pisifera type oil palm. The results show that the treatment using Murasige and Skoog (MS) culture media is able to give a better effect when compared to the use of Eeuwens and Blake (Y3) basic media. While the use of PGR treatment (H6 = NAA 6 mg/l + 2.4-D 0.5 mg/l) showed a callus growth percentage of 76%, which was better than (H1 = Kin 0.1 mg/l + 2.4-D 100 mg/l) by 42%.

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Published

2021-11-03

How to Cite
Write scientific names with Italic fonts:

Sulaksono, G., Hasmeda, M., Hanum, L., Wendra, F. ., Santika, B. ., & Asmono, D. . (2021). THE EFFECT OF CULTURE MEDIA TYPE AND PLANT GROWTH REGULATORS ON CALLUS INDUCTION OF OIL PALM (Elaeis guineensis Jacq) PISIFERA TYPE. BIOVALENTIA: Biological Research Journal, 7(2), 55-60. https://doi.org/10.24233/biov.7.2.2021.224

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Section

Vol 7, No 2 (2021): Nov 2021

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