EXTRACTION AND PURITY DNA OF Culex spp MOSQUITO IN KEMELAK VILLAGE, BINDUNG LANGIT, OGAN KOMERING ULU

Authors

  • Farah Nuriessa Aputri Department of Biosciences Program, Faculty of Science, Sriwijaya University, Jalan Padang Selasa 524, Palem-bang, South Sumatra 30139, Indonesia
  • Laila Hanum Department of Biology Faculty of Mathematics & Natural Sciences, Sriwijaya University. Jalan Raya Palembang-Prabumulih km 32, Indralaya, Indonesia
  • Ayu Safitri Department of Environmental Science, Graduate Program, Sriwijaya University. Jalan Padang Selasa 524, Palembang, South Sumatra 30139, Indonesia
  • Yuanita Windusari Departement of Public Health Mathematics & Natural Sciences, Sriwijaya University. Jalan Raya Palembang-Prabumulih km 32, Indralaya, Indonesia

Article Metrics

This article Abstract has been read: 32 times
PDF Downloads: 28 times
Total Galley Downloads: 28 times
Total Views: 60 times

DOI:

https://doi.org/10.24233/biov.7.2.2021.217

Keywords:

Culex, extraction DNA, moleculer, concentration and purity of DNA

Abstract

Culex spp are mosquito vectors that have a very wide distribution capability and are carriers of pathogens that can interfere with human and animal health. The wide distribution makes Culex spp a dangerous threat. DNA extraction is one of the important steps in obtaining genetic information and genetic analysis. Good quality DNA is used for activities such as the use of molecular markers, genome library creation, and sequencing. This study aims to determine the quality, concentration and purity of Culex spp mosquito DNA in Kemelak Bindung Langit Village, OKU Regency. It is hoped that the sample can be used for further research analysis on Mitochondria D-Loop Sequences in Culex spp mosquitoes. Quantitative measurement of DNA in the form of concentration and purity of DNA using Nanodrop Thermo cycle while qualitative DNA using electrophoresis technique. The results of the isolation of the mosquito genome DNA, obtained clear DNA bands without any degradation (smear) and the concentration results for the four samples ranged from 10-100 ng/µL and the DNA purity was good, ranging from 1.8 to 2.00.

Last Year PDF Downloads

Download data is not yet available.

References

X. Feng, V. L. D. Amo, E. Mameli, M. Lee, A. L. Bishop, N. Perrimon and V. M. Gantz, “Optimized CRISPR tools and site-directed transgenesis towards gene drive development in Culex quinquefasciatus mosquitoes”, Nature Communications, Vol. 12, No. 1, PP. 1-13, 2021.

S. Sabesan, P. Vanamail, K. H. K. Raju, and P. Jambulingam, “Lymphatic filariasis in India: epidemiology and control measures”, Journal Postgrad. Med, Vol. 56, No. 3, PP. 232–238, 2010.

T. G. Andreadis, J. F. Anderson, C. R. Vossbrinck, and A. J. Main, “Epidemiology of West Nile virus in Connecticut: a five-year analysis of mosquito data 1999–2003”, Vector-Borne Zoonotic Dis, Vol. 4, No. 4, PP. 360–378, 2004.

D. M. Fonseca, J. L. Smith, R. C. Wilkerson, and R. C. Fleischer, “Pathways of expansion and multiple introductions illustrated by large genetic differentiation among worldwide populations of the southern house mosquito”, Am. J. Trop. Med. Hyg, Vol. 74, No. 2, PP. 284–289, 2006.

S. N. Okiwelu, and M. A. E. Noutcha, “Breeding sites of Culex quinquefasciatus (Say) during the rainy season in rural lowland rainforest, Rivers State, Nigeria”, Public Health Research, Vol. 2, No. 4, PP. 64–68, 2012.

F. N. Aputri, Y. Windusari, D. Septiawati, L. Hanum, A. Imsya, R. Palupi, and F. N. L. Lubis, “Preference For Landing And Bitting Of Mosquitoes Based On Blood Type: Case study in the Kemelak Bindung Langit Village, Ogan Komering Ulu District, South Sumatra. 7th International Conference-Sustainable Agriculture, Food and Energy (SAFE2019)”, Phuket, Rajabhat University, Thailand, October 18-21, 2019.

Yuwono, Biologi Molekuler. Jakarta: Erlangga, 2005.

M. Dairawan, P. J. Shetty, “The evolution of DNA extraction methods”, America Journal of Biomedical Science and Research, Vol. 8, No. 1, PP. 39-46, 2020.

G. Hoarau, J. A. Coyer, W. T. Stam, J. L. Olsen, “A fast and inexpensive DNA extraction/purification protocol for brown macroalgae”, Molecular Ecology Notes, Vol. 7, No. 2, PP. 191-193, 2007.

Y. Lin, J. Yanhua, L. Qingjiao, S. Zhe, W. Lianzhu, Z. Yuxiu, “A comparison of eight methods for DNA etraction from processed seafood products”, Food Science and Technology Research, Vol. 22, No. 6, PP. 751-757, 2016.

D. Dwiyitno, S. Hoffman, K. Parmentier, C. V. Keer, “Method comparison of DNA isolation and quantification for fish and seafood authenticity determination”, Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Vol. 13, No. 3, PP. 115- 124, 2018.

J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular Cloning Laboratory Manual 3rd Ed. New York: Cold Spring Harbour Lab. Press, 1989.

S. Fatchiyah, E. L. Widyarti, Arumningtyas, and S. Permana, Practical Book of Molecular Biology Analysis Techniques. Malang: Universitas Brawijaya, 2012.

WHO, Mannual on Practical Entomology In Malaria Part II. The WHO Division of Malaria and Other Parasitic Disease, 1976.

C.T. O’connor, and T. Sopa, A Checklist of The Mosquitoes of Indonesia. U.S: Naval Medical Research Unit, 1981.

H. Chen, M. Rangasamy, S. Y. Tan, H. Wang, B. D. Siegfried, “Evaluation of five methods for total DNA extraction from western corn rootworm beetles”, PLoS One, Vol. 5, No. 8, PP. 1-6, 2010.

Westermeier, Electrophoresis in Practice: A Guide to Theory and Practice. New Jersey (US): John Wiley & Sons Inc, 2004.

E. D. Nugroho, D. A. Rahayu, Introduction to biotechnology (theory and application). Yogyakarta: Penerbit Deepublish, 2017.

S. K. Sahu, M. Thangaraj, K. Kathiresan, "DNA Extraction Protocol for Plants with High Levels of Secondary Metabolites and Polysaccharides without Using Liquid Nitrogen and Phenol", International Scholarly Research Notices, Vol. 2012, PP. 1-6, 2012.

M. Alaey, R. Naderi, A. Verzaei, A. Khalighi, A. Salami, “Comparing study between four different methods of genomic DNA extraction from Cyclamen persicum Mill”, International Journal of Agriculture and Biology, Vol. 7, No. 6, PP. 882-884, 2005.

L. Hanum, Y. Windusari, A. Setiawan, Muharni, and F. Adriansyah, “Comparison of CTAB Method and Wizard Genomic DNA Purification System Kit from Promega on DNA Isolation of Local Varities of Rice of South Sumatera”, Science and Technology Indonesia, Vol. 3, No. 1, PP. 24-29, 2018.

A. A. Setiaputri, G. R. Barokah, M. A. B. Sahaba, R. D. Arbajayanti, N. Fabella, R. M. Pertiwi, M. Nurilmala, R. Nugraha, A. Abdullah, “Comparison of DNA isolation methods in fresh and processed fishery products”, Indonesian Journal of Fishery Products Processing, Vol. 23, No. 3, PP. 447-458, 2020.

Kapa Biosystems, 2014, KAPA2G Fast HotStart PCR Kit Technical Data Sheet [internet]. Kapa Biosystems Inc., Wilmington, MA, Available from: https://www.kapabiosystems.com/assets/KAPA2G_Fast_HotSt art_TDS.pdf, 2021

M. Iqbal, D. B. Ibnu, K. Nia, “Comparative analysis of DNA isolation methods for the detection of White Spot Syndrome Virus (WSSV) in Vaname shrimp. (Litopenaeus vannamei)”, Journal of Marine Fisheries. Vol. 8, No. 1, PP. 54-65, 2016.

Published

2021-10-11

How to Cite
Write scientific names with Italic fonts:

Nuriessa Aputri, F., Hanum, L., Safitri, A., & Windusari, Y. (2021). EXTRACTION AND PURITY DNA OF Culex spp MOSQUITO IN KEMELAK VILLAGE, BINDUNG LANGIT, OGAN KOMERING ULU. BIOVALENTIA: Biological Research Journal, 7(2), 50-54. https://doi.org/10.24233/biov.7.2.2021.217

Issue

Section

Vol 7, No 2 (2021): Nov 2021

Most read articles by the same author(s)