ISOLATION AND PURITY DNA FROM LEAF STORAGE OF Lansium domesticum CORRESPOND FOR BARCODING ANALYSIS
DOI:
https://doi.org/10.24233/biov.9.1.2023.251Keywords:
DNA Isolation , DNA Purity , DNA Barcoding , Lansium domesticumAbstract
Lansium domesticum is one of tropical plant. Genetic studies of tropical plant species occurred very slowly due to some obstacles. Provided fresh sample for isolation step was one of impediment. Fresh sample was important conditions to avoid impure DNA template. A simple and rapid technique was needed to get a pure DNA from storage sample. The aims of this study is to see the purity of the DNA isolation results from Lansium domesti-cum mature leaf from South Sumatra that have been stored for 2 years and to test whether the DNA isolation results can be used for barcoding analysis by amplification using PCR method with rbcL primer. There are 11 sample of Lansium domesticum were taken from 8 districts in South Sumatra. The sample was the mature leaves of Lanisum domesticum. The leaves were stored in a freezer at -20oC for two years. There was one sam-ple stored within 2 weeks as a comparison. The results of the study showed the isolation of DNA from storage and mature leaves of Lansium domesticum used Plant Genomic DNA Kit DP305 had a good quantity for 7 sample. 3 sample had low purity and 1 sample contaminated by RNA. Despite electrophoresis showed the isola-tion result not intact and compact, nevertheless the PCR results showed 11 samples could be amplified using rbcL primer. This result indicated the DNA isolation method suit for barcoding analysis using PCR method.
Last Year PDF Downloads
References
T.K. Lim. Edible Medicinal and Non-Medicinal Plants Volume 3, Fruits. Inggris: Springer, 2012. DOI: https://doi.org/10.1007/978-94-007-1764-0
K. S. Yulita, “Genetic variations of Lansium domesticum Corr. accessions from Java, Sumatra and Ceram based on Random Amplified Poly-morphic DNA fingerprints”, Biodiversitas. Vol.I2, No.3, PP. 125-130, 2011. DOI: https://doi.org/10.13057/biodiv/d120301
Rupiah, L. Hanum, Z. P. Negara, Z. Dahlan, and I. Yustian, “Morphological Diversity of Lansium domesticum Corr in South Sumatera”, Science and Technology Indonesia. Vol.3. No.1. PP. 41-44. 2018. DOI: https://doi.org/10.26554/sti.2018.3.1.41-44
M. Restu, Mukrimin and Gusmiaty, “Optimalisasi Teknik Ekstraksi dan Isolasi DNA Suren (Toona sureni Merr.) untuk Analisis Keragaman Genetik berdasarkan Random Amplified Polymorphic DNA (RAPD)”, Jurnal Natur Indonesia. Vol.14, No.2. 138-142, 2012 DOI: https://doi.org/10.31258/jnat.14.1.138-142
F. Hindayani, R. A. Wulandari and R.H. Murti, “Genomic DNA Extraction Method from Mayure Leaf of Lai (Durio kutejensis Becc.)”, Agrivita. Vol. 38, No. 1, PP. 73-79, 2016.
S.K. Sahu, M. Thangaraj and K. Kathiresan, “DNA Extraction Protocol for Plants with High Level of Secondary Metabolites and Polysaccha-rides without Using Liquid Nitrogen and Phe-nol”, International Scholary Research Notices, Vol.2012, PP. 1-6, 2012. DOI: https://doi.org/10.5402/2012/205049
N.A. Aboul-Maaty and H. A. Oraby, “Extraction of high-quality genomic DNA from Different Plant Orders Applying a Modified CTAB-based Method”. Bulletin of National Research Centre, Vol.43, No.25. PP. 1-10, 2019. DOI: https://doi.org/10.1186/s42269-019-0066-1
Y. Shavrokov, N. Borisjuk and N. K. Gupta, “Plant Genetics and gene Study”, BioMed Re-search International. Vol. 2019. PP. 1-2, 2019. DOI: https://doi.org/10.1155/2019/3560374
S. C. Tan and B. C. Tiap, “DNA, RNA and Protein Extraction: The Past and The Present”, Journal of Biomedicin and Biotechnology, Vol. 2009. PP. 1-10, 2009. DOI: https://doi.org/10.1155/2009/574398
M. S. A. Tuffaha. Phenotypic and Genotypic Diagnosis of Malignancies an Immunohisto-chemical and Molecular Approach. German: Wiley Publishers, 2008. DOI: https://doi.org/10.1002/9783527621521
P.Y. Lee, J. Costumbrado, C. Y. Hsu and Y. H. Kim, “Agarose Gel Electrophoresis for The Separation of DNA Fragments”, Journal of Vis-ualized Experiment. No.62. PP. 1-5. 2012. DOI: https://doi.org/10.3791/3923-v
W.J. Kress dan D. L. Erickson, “A Two-Locus Global DNA Barcode for Land Plants: The Coding rbcL Gene Complements the Non-Coding trnH-psbA Spacer Region”, PLoS ONE. Vol. 2. No.6. PP. 1-10, 2007. DOI: https://doi.org/10.1371/journal.pone.0000508
E. Harnelly, Z. Thomy and N. Fathiya, “Phy-logenetic Analysis of Dipterocarpaceae in Ket-ambe Research Station, Gunung Leuser National Park (Sumatera, Indonesia) based on rbcL and matK gene”, Biodiversitas. Vol.19. No.3. PP. 1074-1080, 2018. DOI: https://doi.org/10.13057/biodiv/d190340
R. E. Farrel. RNA Methodologies a Laboratory Guide for Isolation and Characterization Fourth Edi-tion. America: Elsevier Science, 2009.
J. T. Keer and L. Brich. Essentials of Nucleic Acid Analysis a Robust Approuch. UK: Royal Society of Chemistry, 2008. DOI: https://doi.org/10.1039/9781847558213
S. M. M. Nzilibili, M. K. H. Ekodiyanto, P. Hardjanto and A. Yudianto, “Concentration and Purity DNA Spectrophotometer: Sodium Mono-fluorophosphate Forensic Impended Effect”, Egyptian Journal of Forensic Sciences. Vol.8. No. 34. PP. 1-7, 2018. DOI: https://doi.org/10.1186/s41935-018-0065-7
Y. Xia, F. Chen, T. Du, C. Liu, G. Bu, Y. Xin and B. Liu, “a Modified SDS-based DNA Ex-traction Method from Raw Soybean”, Biosci-ence Report. Vol.39. PP. 1-10, 2022. DOI: https://doi.org/10.1042/BSR20182271
O. Nath, S. J. Fletcher, A. Hayward, L. M. Shaw, R. Agarwal, A. Furtado, R. J. Henry and N. Mitter, “A Comprehensive High-Quality DNA and RNA Extraction Protocol for a Range of Cultivars and Tissue Types of The Woody Crop Avocado”, Plants. Vol.11. No.242. 1-17. 2022. DOI: https://doi.org/10.3390/plants11030242
V. Martida and M. Pharmawati, “Comparison of DNA Yield from Different Plant Materials of Plumeria sp. (Apocynaceae)”, Journal of Ad-vances in Tropical Biodiversity and Environ-mental Science. Vol.3. No.1. 8-11. 2019. DOI: https://doi.org/10.24843/ATBES.2019.v03.i01.p03
P.A. Moreira and D. A. Oliveira, “Leaf Age Affects the Quality of DNA Extracted from Di-morphandra mollis (Fabaceae), a Tropical Tree Species from The Cerrado Region of Brazil”, Genetic and Molecular Reasearch. Vol.10. No.1. PP. 353-358, 2011. DOI: https://doi.org/10.4238/vol10-1gmr1030
T. Wilkes. DNA Techniques to Verify Food Authenticity: DNA Extraction from Food Ma-trix. UK:Royal Society of Chemistry. 2019. DOI: https://doi.org/10.1039/9781788016025-00029
F. Handayani, R. A Wulandari and R. H. Murti, “Genomic DNA Extraction Method for Mature Leaf of Lai (Durio kutejensis Becc.)”, Agrivita. Vol.38. No.1. PP. 73-79, 2016. DOI: https://doi.org/10.17503/agrivita.v38i1.659
V. K. Sari and R. H Murti, “An Effective Method for DNA Extraction of Mature Leaf of Sapondilla (Manikara zapota (L,) van Royen)”, Agrivita. Vol 37 No.1. PP. 18-23, 2015 DOI: https://doi.org/10.17503/Agrivita-2015-37-1-p018-023
D.A. G Perwitasari, S. Rohimah, T. Ratnasari, B. Sugiharto and M. Su’ud, “DNA Barcoding of Medical Orchid Dendrobium dis-color Lindl. Tanimbar Using rbcL and ITS Gene”, Buletin Penelitian Tanaman Rempah dan Obat. Vol 31 No.1. PP. 8-20, 2020. DOI: https://doi.org/10.21082/bullittro.v31n1.2020.8-20
H. Shabrina, U. J. Siregar, D. D. Matra, K. Kamiya and I. Siregar, “Short Communication: DNA Extraction from Stored Wood of Falca-taria moluccana Suitable for Barcoding Anal-yasis”, Biodiversitas. Vol.20. No.6. PP. 148-153, 2019. DOI: https://doi.org/10.13057/biodiv/d200635
R. B. L Koh, C.F.C. Barbosa, V. M. Aquino, and L. C. Galvez, “Extraction of High Molecular Weight DNA Suitable for Next-Generation Se-quencing from The Fiber Crop Abaca”, Indus-trial Crops and Products. Vol.161. PP. 1-9, 2021. DOI: https://doi.org/10.1016/j.indcrop.2020.113194
L. Zang, B. Wang, L. Pan, and J. Peng, “Re-cycling Isolation of Plant DNA, A Novel Meth-od”, Journal of Genetics and Genomics. Vol.40. PP. 45-54, 2013. DOI: https://doi.org/10.1016/j.jgg.2012.10.001
P. W. Inglis, M. D. C. R. Pappas, L. V. Resende and D. Grattapaglia, “Fast and Inex-pensive Protocols for Consistent Extraction of High-Quality DNA and RNA from Challenging Plant and Fungal Samples for High-Throughput SNP Genotyping and Sequencing Applications”, Plos One. Vol.13. No.10. PP. 1-14, 2018. DOI: https://doi.org/10.1371/journal.pone.0206085
Y. Rachmawati and R. A. Khiriyah, “Com-parison of DNA Isolation Result using Simple Methods and Kits in Samples of Psidium guaja-va Leaves”, Biotropic the Journal of Tropical Biology. Vol.2. No.2. PP. 94-99, 2018. DOI: https://doi.org/10.29080/biotropic.2018.2.2.93-99
L. Hanum, Y. Windusari, A. Setiawan, Mu-harni, F. Adriansyah and A. A. Mubarok, “Com-parison of CTAB Method and Wizard Genomic DNA Purification System Kit for Promega on DNA Isolation of Local Varieties of Rice of South Sumatera”, Science and Technology Indo-nesia. Vol.3. PP. 26-29,2018. DOI: https://doi.org/10.26554/sti.2018.3.1.26-29
PDF Downloads: 909
Published
How to Cite
Write scientific names with Italic fonts:
Issue
Section
Copyright (c) 2023 Nur Arifah, Laila Hanum, Sarno Sarno, Nita Aminasih, Singgih Tri Wardana, Hary Widjajanti
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
d. Authors hold the copyright and retain publishing right of articles without restrictions.
.gif)
