POTENCY AND ACTIVITY OF SECONDARY METABOLITE OF Trichoderma harzianum AC1(b) J2 INHIBITOR GROWTH Colletotrichum capsici IPBCC 13.1098
DOI:
https://doi.org/10.24233/BIOV.5.1.2019.130Keywords:
Secondary metabolites , Trichoderma harzianum , Colletotrichum capsici , Alkaloite , Scleria poaeformisAbstract
Trichoderma harzianum is a fungus that can produce secondary metabolites which able to inhibit the growth of Colletotrichum capsici. a pathogenic fungus causing anthracnose in plants. the Aims of this research were to obtain and analyze the ability of secondary metabolites of Trichoderma harzianum AC1 (b) J2 isolated from Scleria poaeformis. to inhibit the growth of Colletotrichum capsici IPBCC13.1098. The secondary metabolite eluat of Trichoderma harzianum were tested for the antifungal activity toward Colletotrichum capsici IPBCC 13.1098. Fractionation was carried out by Column Chromatography and produced 31 eluat. The secondary metabolite eluat of Trichoderma harzianum with the highest inhibition zone diameter was eluat 1 with 8.4 mm in diameter. The secondary metabolite eluat with the highest value of inhibition zone was carried out by MIC test and thin layer chromatography (TLC). Based on the MIC results, the minimum inhibitory concentration of the secondary metabolite of Trichoderma harzianum to fungus Colletotrichum capsici was 250 ppm with inhibition zone diameter was 0.10 mm. The results of TLC showed orange spots on the TLC plate that indicated alkaloite compounds.
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References
M. Agen, H. Trichodermaharzianum, and D. A. N. Actinomycetes, “Supriati, L., dkk Pengendalian Penyakit Antraknosa pada ….,” pp. 20–26, 2016.
dan L. D. Asniah, Dian Lestari, Mariadi, “Potensi cendawan endofit nonpatogen asal akar tanaman cabai (,” Agriplus, vol. 24, pp. 177–183, 2014.
F. Vinale et al., “Trichoderma Secondary Metabolites Active on Plants and Fungal Pathogens,” Open Mycol. J., vol. 8, no. 1, pp. 127–139, 2014.
J. Taribuka et al., “Eksplorasi dan identifikasi,” vol. 16, no. 2, pp. 115–123, 2016.
S. Kalaiselvi and A. Panneerselvam, “INTERNATIONAL JOURNAL OF PHARMACEUTICS & DRUG ANALYSIS Bioactive Compound Produced By Trichoderma harzianum For Control- ling Sarocladium oryzae,” vol. 6, no. 2, pp. 248–251, 2018.
M. M. Afandi, S. F. Sitepu, and Lisnawita, “Potensi Trichoderma spp. Asal Rizosfer Tanaman Kelapa Sawit sebagai Agens Antagonis Terhadap Ganoderma sp. secara in vitro,” J. Agroekoteknologi FP USU, vol. 5, no. 2, pp. 469–473, 2017.
N. R. Bhardwaj and J. Kumar, “Characterization of volatile secondary metabolites from Trichoderma asperellum,” vol. 9411, 2017.
M. Agen et al., “Major secondary metabolites produced by two commercial Trichoderma strains active against different phytopathogens,” Lett. Appl. Microbiol., vol. 43, no. 2, pp. 143–148, 2006.
M. Chatri, “Pengaruh Media (Campuran Beras Dan Ampas Tebu) terhadap Pertumbuhan Trichoderma harzianum dan Daya Hambatnya terhadap Fusarium oxysporum secara In vitro,” Bioscience, vol. 2, no. 1, p. 50, 2018.
M. Agen et al., “Artikel Penelitian Uji Aktivitas Antijamur Ekstrak Etanol Umbi Bawang Dayak ( Eleutherine americana ( Aubl .) Merr . Ex K . Heyne .) terhadap Trichophyton mentagrophytes secara In Vitro,” J. Berk. Perikan. Terubuk, vol. 2, no. 1, pp. 20–26, 2016.
F. Vinale, R. Marra, F. Scala, E. L. Ghisalberti, M. Lorito, and K. Sivasithamparam, “Major secondary metabolites produced by two commercial Trichoderma strains active against different phytopathogens,” Lett. Appl. Microbiol., vol. 43, no. 2, pp. 143–148, 2006.
S. Desmara et al., “No Title,” vol. 2, no. Februari, pp. 31–39, 2017.
U. M. S. andfa@yahoo. co. Andi Suhendi*, Landyyun Rahmawan Sjahid, Dedi Hanwar Fakultas Farmasi and ABSTRAK, “ISOLATION AND IDENTIFICATION OF FLAVONOIDS FROM DEWANDARU (Eugenia uniflora L.) LEAF,” EnANPAD 2006, vol. 12, no. 2, pp. 1–16, 2006.
Salni, N. Aminasih, and R. Sriviona, “Isolasi Senyawa Antijamur Dari Rimpang Lengkuas Putih ( Alpinia galanga ( L .) Willd ) Dan Penentuan Konsentrasi Hambat Minimum Terhadap Candida albicans,” Pros. Semirata FMIPA Univ. Lampung, pp. 301–308, 2013.
F. Indrayati, M. A. Wibowo, and N. Idiawati, “AKTIVITAS ANTIJAMUR EKSTRAK DAUN SAGA POHON ( Adenanthera Pavonina L .) TERHADAP JAMUR Candida albicans,” Jkk, vol. 5, no. 2, pp. 20–26, 2016.
P. Metabolit et al., “Chimica et Natura Acta,” vol. 5, no. 2, pp. 85–89, 2017.
D. ∙ Y. P. Kusbiantoro, “Pemanfaatan kandungan metabolit sekunder pada tanaman kunyit dalam mendukung peningkatan pendapatan masyarakat Utilization of secondary metabolite in the turmeric plant to increase community income,” Kultivasi, vol. 17, no. 1, pp. 544–549, 2018.
V. E. Mariana, E. Liviawaty, and I. D. Buwono, “Penyiapan bahan penunjang : Penyiapan daging lele 100 gr : Pengisian ke dalam selongsong dan pengikatan,” J. Perikan. dn Kelaut., vol. 2, no. 1, pp. 73–79, 2011.
E. Chasanah, N. M. Noor, Y. Risjani, and A. S. Dewi, “Aktivitas Antibakteri Dan Antioksidan Ekstrak Streptomyces sp. dan Exserohilum rostratum yang Dikultivasi Pada Tiga Jenis Medium Pertumbuhan,” J. Pascapanen dan Bioteknol. Kelaut. dan Perikan., vol. 7, no. 1, p. 39, 2017.
A. Elita, S. Saryono, and J. Christine, “Penentuan Waktu Optimum Produksi Antimikroba dan Uji Fitokimia Ekstrak Kasar Fermentasi Bakteri Endofit Pseudomonas sp. dari Umbi Tanaman Dahlia (Dahlia variabilis),” J. Ind. Che Acta, vol. 3, no. 2, pp. 56–62, 2013.
F. Firdayani and T. Winarni Agustini, “Ekstraksi Senyawa BIoaktif sebagai Antioksidan Alami Spirulina Platensis Segar dengan Pelarut yang Berbeda,” J. Pengolah. Has. Perikan. Indones., vol. 18, no. 1, pp. 28–37, 2015.
M. Mukhriani, A. Ismail, H. Haeria, S. Syakri, and N. Fadiyah, “IDENTIFIKASI GOLONGAN SENYAWA ANTIBAKTERI FRAKSI POLAR DAN NON POLAR KULIT BATANG KEMIRI (Aleurites moluccana L. Willd) DENGAN METODE BIOAUTOGRAFI KONTAK,” J. Farm. UIN Alauddin Makassar, vol. 6, no. 1, pp. 47–54, 2018.
D. Ganoderma, “-1 . -1 ,” pp. 190–198.
dan M. A. Sumarto, Desmelati, Dahlia, Bustari Hasan, “Penentuan Senyawa Bioaktif Ekstrak Daging Siput Bakau (Terebralia sulcata) dengan Kromatografi Lapis Tipis (KLT),” J. Berk. Perikan. Terubuk, vol. 39 (2), no. Juli 2011, pp. 85–96, 2011.
N. Afni, N. Said, and Yuliet, “Uji Aktifitas Antibakteri Pasta Gigi Ekstrak Biji Pinang ( Areca catechu L . ) Terhadap Streptococcus mutans dan Staphylococcus aureus,” Galen. J. Pharm. ISSN 2442-8744, vol. 1, no. March, pp. 48–58, 2015.
Mursadam, Wildan, and A. Ramdani, “Jurnal Penelitian Pendidikan IPA ( JPPIPA ),” J. Penelit. Pendidik. IPA, vol. 3, no. 2, 2017.
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