CELLULASE ACTIVITY OF RIZOSFER BACTERIA OF KETAPANG PLANTS (Terminalia catappa L.)

Authors

  • Rosmania Rosmania Student Magister Biologi, Faculty of Mathematic & Natural Science, Sriwijaya University. Jalan Padang Selasa No. 524, Palembang, Indonesia
  • Hary Widjajanti Department of Biology, Faculty of Mathematics & Natural Sciences, Sriwijaya University. Jalan Raya Palem-bang-Prabumuliuh km 32, Indralaya, Indonesia.
  • Elisa Nurnawati Department of Biology, Faculty of Mathematics & Natural Sciences, Sriwijaya University. Jalan Raya Palem-bang-Prabumuliuh km 32, Indralaya, Indonesia

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DOI:

https://doi.org/10.24233/biov.9.2.2023.403

Keywords:

Cellulase , Cellulolytic Bacteria , Rhizosphere , Carboxy Methyl Cellulose , DNS method

Abstract

Cellulose, a natural biopolymer produced by plants, undergoes enzymatic breakdown by cellulase, cleaving the monomer's β-1,4 glycosidic bonds. Cellulolytic bacteria, producers of cellulase, can be isolated from plant rhizospheres, like Ketapang (Terminalia catappa L.), abundantly found in the Biology Department at Universitas Sriwijaya, South Sumatra, Indonesia. This study aimed to isolate cellulolytic bacteria from Ketapang rhizosphere soil and assess their cellulase activity. The spread plate method on selective Carboxy Methyl Cellulose Agar (CMC agar) was used for bacterial isolation, and cellulase activity was measured using the DNS method (3.5-dinitro salicylic acid). Results yielded 22 isolates of cellulolytic bacteria capable of growth on selective CMC agar. Thirteen isolates, identified through a screening test, produced cellulase, forming clear zones post-addition of 0.1% Congo Red and 1 M NaCl. Cellulolytic activity indices for these isolates ranged from 0.02 to 2.6 mm. The highest indices (IAS) and cellulase activities were observed in BS10, BS4, and BS22, with IAS values of 2.64, 2.12, and 1.71, and cellulase activities of 354.99 U/ml, 9.42 U/ml, and 9 U/ml, respectively. Identification results suggested that isolate BS10 bore similarities to the genus Zoogloea, while isolates BS4 and BS22 showed similarities to the genus Bacillus.

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References

F. Razie, I. Anas, A. Sutandi, L. Gunarto, and S. Sugiyanta, “Aktivitas enzim selulase mikroba yang diisolasi dari jerami padi di persawahan pasang surut di Kalimantan Selatan,” J. Ilmu Tanah dan Lingkung., vol. 13, no. 2, p. 43, 2011, doi: 10.29244/jitl.13.2.43-48.

H. Murtiyaningsih and M. Hazmi, “Isolasi dan uji aktivitas enzim selulase pada bakteri selulolitik asal tanah sampah isolation and cellulase enzyme activities assays in cellulolytic bacteria origin from soil waste,” Agritrop, vol. 15, no. 2, pp. 293–308, 2017, [Online]. Available: http://jurnal.unmuh jember.ac.id/

M. Nababan, I. B. W. Gunam, and I. M. Mahaputra Wijaya, “Produksi enzim selulase kasar dari bakteri selulolitik,” J. Rekayasa dan Manaj. Agroindustri, vol. 7, no. 2, p. 190, 2019, doi: 10.24843/jrma.2019.v07.i02.p03.

P. Gupta, K. Samant, and A. Sahu, “Isolation of cellulose-degrading bacteria and determination of their cellulolytic potential,” Int. J. Microbiol., vol. 2012, 2012, doi: 10.1155/2012/578925.

S. Acharya and A. Chaudhary, “Bioprospecting thermophiles for cellulase production: A review,” Brazilian J. Microbiol., vol. 43, no. 3, pp. 844–856, 2012, doi: 10.1590/S1517-83822012000300001.

A. G. Rahayu, Y. Haryani, and F. Puspita, “uji aktivitas selulolitik dari tiga isolat bakteri Bacillus sp. galur lokal riau ariani,” Jom Fmipa, vol. 1, no. 2, pp. 319–327, 2014.

Yu NN, Ketya W, Park G. Intracellular Nitric Oxide and cAMP Are Involved in Cellulolytic Enzyme Production in Neurospora crassa. Int J Mol Sci. 2023 Feb 24;24(5):4503.

S. A. Ladeira, E. Cruz, A. B. Delatorre, J. B. Barbosa, and M. L. L. Martins, “Cellulase production by thermophilic Bacillus sp. SMIA-2 and its detergent compatibility,” Electron. J. Biotechnol., vol. 18, no. 2, pp. 110–115, 2015, doi: 10.1016/j.ejbt.2014.12.008.

S. Sethi, A. Datta, B. L. Gupta, and S. Gupta, “Optimization of Cellulase Production from Bacteria Isolated from Soil,” ISRN Biotechnol., vol. 2013, pp. 1–7, 2013, doi: 10.5402/2013/985685.

A. Hartmann, M. Schmid, D. van Tuinen, and G. Berg, “Plant-driven selection of microbes,” Plant Soil, vol. 321, no. 1–2, pp. 235–257, 2009, doi: 10.1007/s11104-008-9814-y.

E. Widyati, “Understanding Underground-Plants Communication to Optimize Land Management,” J. Sumberd. Lahan, vol. 11, no. 1, pp. 33–42, 2017.

D. Istarina, S. Khotimah, and M. Turnip, “Aktivitas Antibakteri Ekstrak Metanol Buah Ketapang (Terminalia catappa Linn.) Terhadap Pertumbuhan Staphylococcus epidermidis Dan Salmonella typhi,” Protobiont, vol. 4, no. 3, pp. 98–102, 2015.

I. Mulyadi, “Isolasi Dan Karakteristik Selulosa,” J. Saintika Unpam, vol. 1, no. 2, pp. 177–180, 2019.

A. A. Ed-Har, R. Widyastuti, and G. Djajakirana, “Isolasi dan Identifikasi Mikroba Tanah Pendegradasi Selulosa dan Pektin dari Rhizosfer Aquilaria malaccensis,” Bul. Tanah dan Lahan, vol. 1, no. 1, pp. 58–64, 2017.

D. N. Islamiah, R. Linda, and Rahmawati, “Jenis-jenis Bakteri Rizosfer Kawasan Tanah Mangrove Avicennia di Kelurahan Terusan, Kecamatan Mempawah Hilir, Kalimantan Barat,” J. Protobiont, vol. 6, no. 3, pp. 165–172, 2017.

S. A. Bhagat and S. S. Kokitkar, “Isolation and identification of bacteria with cellulose-degrading potential from soil and optimization of cellulase production,” J. Appl. Biol. Biotechnol., vol. 9, no. 6, pp. 154–161, 2021, doi: 10.7324/JABB.2021.96020.

S. Naresh, B. Kunasundari, A. Anas, N. Gunny, and Y. Peng, “Isolation and Partial Characterisation of Thermophilic Cellulolytic Bacteria from North Malaysian Tropical Mangrove Soil Mangrove is an ecosystem that protects the land along the seashore from the soil erosion . It is also associated with the defense of s,” vol. 30, no. 1, pp. 123–147, 2019.

Y. W. Choi, I. J. Hodgkiss, and K. D. Hyde, “Enzyme production by endophytes of Brucea javanica,” Water, pp. 55–66, 2005.

S. I. Fauziah and M. Ibrahim, “Isolasi dan Karakterisasi Bakteri Selulolitik pada Tanah Gambut di Desa Tagagiri Tama Jaya, Kecamatan Pelangiran, Kabupaten Inhil, Riau,” LenteraBio Berk. Ilm. Biol., vol. 9, no. 3, pp. 194–203, 2021, doi: 10.26740/lenterabio.v9n3.p194-203.

C. Kumar Gautam and A. Prem Rajan, “Isolation and Screening of Cellulolytic Bacteria Inhabiting Different Environment and Optimization of Cellulase Production,” Environmentaljournal.Org, vol. 3, no. 1, pp. 39–49, 2249, [Online]. Available: https://www.environmentaljournal.org/4-2/ujert-4-2-5.pdf

D. Puspitasari and M. Ibrahim, “Optimasi Aktivitas Selulase Ekstraseluler Islolat Bakter EG 2 Isolasi dari Bungkil Kelapa Sawit (Elaesis guineensis jacq.) Optimization of EExtracelluler Cellulase Activity of Isolate Bacterial EG 2 Isolated from Palm Oilcake (Elaesis guineensis jacq.),” LenteraBio, vol. 9, no. 1, pp. 42–50, 2020, [Online]. Available: https://journal.unesa.ac.id/index.php/lenterabio/index42

N. Hasanah, “Aktivitas selulase isolat jamur dari limbah media tanam jamur merang,” vol. 1, no. Imas 2009, pp. 1110–1115, 2015, doi: 10.13057/psnmbi/m010524.

M. Mulyasari, W. Widanarni, M. A. Suprayudi, M. Z. Junior, and M. T. D. Sunarno, “Seleksi dan Identifikasi Bakteri Selulolitik Pendegradasi Daun Singkong (Manihot esculenta) yang Diisolasi dari Saluran Pencernaan Ikan Gurame (Osphronemus gouramy),” J. Pascapanen dan Bioteknol. Kelaut. dan Perikan., vol. 10, no. 2, p. 111, 2015, doi: 10.15578/jpbkp.v10i2.271.

Thapa. S, Mishra. J, Arora. N, Mishra. P, Li. H, O′Hair. J, Bhatti. S, Zhou S. Microbial cellulolytic enzymes: diversity and biotechnology with reference to lignocellulosic biomass degradation. Rev Environ Sci Biotechnol, vol. 19, no. 621–648, 2020.

Vives-Peris. V, de Ollas. C, Gómez-Cadenas. A, Pérez-Clemente. RM. Root exudates: from plant to rhizosphere and beyond. Plant Cell Rep. vol 39, no. 3–17, 2020.

Z. Arifin, I. B. W. Gunam, N. S. Antara, and Y. Setiyo, “Isolasi Bakteri Selulolitik Pendegradasi Selulosa Dari Kompos,” J. Rekayasa Dan Manaj. Agroindustri, vol. 7, no. 1, p. 30, 2019, doi: 10.24843/jrma.2019.v07.i01.p04.

A. P. Raharjo and Isnawati, “Isolasi dan karakterisasi bakteri selulolitik pada pakan fermentasi eceng gondok, tongkol jagung, dan bekatul padi isolation and characterization cellulolytic bacteria from fermentation feed of water hyacinth, corncob, and rice bran,” Lentera, vol. 11, no. 1, pp. 44–51, 2022, [Online]. Available: https://journal.unesa.ac.id/index.php/lenterabio/index44

A. Pesrita, T. Marta Linda, S. Devi, and J. Biologi Fakultas Matematika Ilmu pengetahuan Alam Universitas Riau, “Seleksi dan Akivitas Enzim Selulase Aktinomisetes Lokal Riau pada Media Lignoselulosa Ampas Tebu,” J. Riau Biol., vol. 2, no. 1, pp. 8–13, 2018, [Online]. Available: http://jurnalunri.org/ojs/index.php/JRB

T. Purwadaria, P. Marbun, A. Sinurat, and P. Ketaren, “Perbandingan Aktivitas Enzim Selulase dari Bakteri dan Kapang Hasil Isolasi dari Rayap,” Jitv, vol. 8, no. 4, pp. 213–219, 2003.

Y. H. Pratiwi, O. Ratnayani, and I. N. Wirajana, “Perbandingan Metode Uji Gula Pereduksi Dalam Penentuan Aktivitas ?-L-Arabinofuranosidase Dengan Substrat Janur Kelapa (Cocos Nucifera),” J. Kim., p. 134, 2018, doi: 10.24843/jchem.2018. v12.i02.p07.

M. Nisa, F. Aini, and H. U. Maritsa, “Aktivitas Antagonistik Bakteri Selulolitik Asal Rhizosfer Kelapa Sawit (Elaeis guineensis Jacq.) terhadap Ganoderma boninense Pat.,” Al-Kauniyah J. Biol., vol. 13, no. 1, pp. 9–19, 2020, doi: 10.15408/kauniyah.v13i1.11704.

Published

27-12-2023

How to Cite
Write scientific names with Italic fonts:

Rosmania, R., Widjajanti, H., & Nurnawati, E. (2023). CELLULASE ACTIVITY OF RIZOSFER BACTERIA OF KETAPANG PLANTS (Terminalia catappa L.). BIOVALENTIA: Biological Research Journal, 9(2), 129–136. https://doi.org/10.24233/biov.9.2.2023.403

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Section

Vol 9, No 2 (2023): Nov 2023