POTENCY OF ENDOPHYTIC FUNGI ISOLATED FROM Muntingia calabura AS ANTIFUNGAL SUBSTANCES AGAINST Candida parapsilosis
Keywords:Muntingia calabura; Candida parapsilosis; Isolate; Antifungal
Alternative is needed to overcome the problem of dandruff caused by C. parapsilosis. Endophytic fungi isolated from plants are capable of synthesizing compounds to produce various secondary metabolites. This study aimed to obtain the most active endophytic fungal isolates, determine the class of compounds, and determine the antifungal activity of the endophytic fungi (M. calabura) against C. parapsilosis. Fifteen isolates of endophytic fungi isolated from M. calabura were coded FDK1-FDK15. There was a color change in the media after 30 days of cultivation isolates of fungi. FDK4 and FDK13 have potential as antifungals. FDK13 fungal extract had the highest inhibition zone diameter of 20.00 mm. 20 eluates were obtained from the isolation of com-pounds (Thin Layer Chromatography), FDK 131, 1314, 1317, and 1320 eluates are compounds of the terpenoid group and FDK1316 eluates are phenol group compounds that are active in inhibiting yeast growth. FDK1317 was the most active compound isolate in inhibiting the growth of C. parapsilosis with an inhibition zone diameter of 12.5 mm.
Last Year PDF Downloads
Ridawati, B. S. L. Jenie, I. Djuwita, and W. Sjamsuridzal, “Aktivitas Antifungal Minyak Atsiri Jinten Putih terhadap Candida parapsilosis SS25, C. orthopsilosis NN14, C. metapsilosis MP27, dan C. etchellsii MP18,” MAKARA Sci. Ser., vol. 15, no. 1, pp. 58–62, 2011.
M. Kao et al., “The mPEG-PCL Copolymer for Selective Fermentation of Staphylococcus lugdunensis Against Candida parapsilosis in the Human Microbiome,” J. Microb. Biochem. Technol., vol. 8, no. 4, pp. 259–265, 2016.
R. J. Hay and G. Midgley, “Introduction: Malassezia Yeast from a Historical Perspective,” in Malassezia and the Skin, 1st ed., T. Boekhout, E. Gueho, P. Mayser, and A. Velegraki, Eds. Heidelberg: Springer, 2010, pp. 1–16.
R. Tóth et al., “Candida parapsilosis: From genes to the bedside,” Clin. Microbiol. Rev., vol. 32, no. 2, pp. 1–38, 2019.
M. Ibrahim et al., “Antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of markhamia tomentosa,” Pharm. Biol., vol. 55, no. 1, pp. 590–595, 2017.
N. Premjanu, C. Jaynthy, and S. Diviya, “Antifungal activity of endophytic fungi isolated from Lannea coromandelica–an insilico approach,” Int. J. Pharm. Pharm. Sci., vol. 8, no. 5, pp. 207–210, 2016.
J. Yu, Y. Wu, Z. He, M. Li, K. Zhu, and B. Gao, “Diversity and antifungal activity of endophytic fungi associated with Camellia oleifera,” Mycobiology, vol. 46, no. 2, pp. 85–91, 2018.
D. A. Putri and S. Fatmawati, “Metabolit Sekunder dari Muntingia calabura dan Bioaktivitasnya,” ALCHEMY J. Penelit. Kim., vol. 15, no. 1, pp. 57–78, 2019.
A. Kurniawati, A. Mashartini, and I. S. Fauzia, “Perbedaan Khasiat Anti Jamur antara Ekstrak Etanol Daun Kersen ( Muntingia calabura L . ) dengan Nistatin terhadap Pertumbuhan Candida albicans,” J. PDGI, vol. 65, no. 3, pp. 74–77, 2016.
W. P. C. Buhian, R. O. Rubio, D. L. V. Junior, and J. J. Martin-puzon, “Bioactive metabolite profiles and antimicrobial activity of ethanolic extracts from Muntingia calabura L. leaves and stems,” Asian Pac. J. Trop. Biomed., vol. 6, no. 8, pp. 682–685, 2016.
C. Leiwakabessy and Y. Latupeirissa, “Eksplorasi Bakteri Endofit sebagai Agens Hayati pada Tanaman Kersen ( Muntingia calabura L .),” J. Budid. Pertan., vol. 9, no. 1, pp. 16–21, 2013.
G. A. Widayanti, H. Widjajanti, and Salni, “Indonesian Journal of Environmental Management and Sustainability Antibacterial Activity of The Secondary Metabolites Produced by Endophytic Fungi Isolated from Acanthus ilicifolius Plant,” Indones. J. Environ. Manag. Sustain., vol. 3, no. 2019, pp. 107–115, 2019.
Ambarwati, T. A. Sujono, and R. Sintowati, “Kombinasi Ekstrak Daun Pandan Wangi ( Pandanus amaryllifolius Roxb.) dan Buah Mengkudu ( Morinda citrifolia ) sebagai Antifungi terhadap Jamur Penyebab Ketombe,” J. Ilmu Kefarmasian Indones., vol. 15, no. 1, pp. 96–101, 2017.
Elfita, Mardiyanto, Fitrya, J. E. Larasati, H. Widjajanti, and Muharni, “Antibacterial activity of Cordyline fruticosa leaf extracts and its endophytic fungi extracts,” Biodiversitas, vol. 20, no. 12, pp. 3804–3812, 2019.
Nurkayah, E. Nurnawati, and H. Widjajanti, “Potency and Activity of Secondary Metabolite of Trichoderma harzianum AC1(b) J2 inhibitor growth Colletotrichum capsici IPBCC 13.1098,” Biovalentia, vol. 5, no. 1, pp. 38–44, 2019.
Salni, H. Marisa, and R. W. Mukti, “Isolasi Senyawa Antibakteri Dari Daun Jengkol ( Pithecolobium lobatum Benth) dan Penentuan Nilai KHM-nya,” J. Penelit. Sains, vol. 14, no. D, pp. 38–41, 2011.
N. Azzahra, M. Jamilatun, and A. Aminah, “Perbandingan Pertumbuhan Aspergillus fumigatus pada Media Instan Modifikasi Carrot Sucrose Agar dan Potato Dextrose Agar,” J. Mikol. Indones., vol. 4, no. 1, pp. 168–174, 2020.
Nurbaya, T. Kuswinanti, Baharuddin, A. Rosmana, and S. Millang, “Uji kecepatan pertumbuhan Fusarium spp. pada media organik dan media sintesis,” J. Bionature, vol. 15, no. 1, pp. 45–53, 2011.
K. M. VanderMolen, H. A. Raja, T. El-Elimat, and N. H. Oberlies, “Evaluation of culture media for the production of secondary metabolites in a natural products screening program,” AMB Express, vol. 3, no. 71, pp. 1–7, 2013.
S. K. Deshmukh, M. K. Gupta, V. Prakash, and S. Saxena, “Endophytic fungi: A source of potential antifungal compounds,” J. Fungi, vol. 4, no. 3, pp. 1–42, 2018.
R. Singh et al., “Phytochemical Analysis of Muntingia calabura Extracts Possessing Anti-Microbial and Anti-Fouling Activities,” Int. J. Pharmacogn. Phytochem. Res., vol. 9, no. 6, pp. 826–832, 2017.
M. Setyaningrum and E. Cahyono, “Pemisahan Sitronelal Menggunakan Kromatografi Kolom dengan Fasa Diam Siklodekstrin Terasetilasi,” Indones. J. Chem. Sci., vol. 5, no. 2, pp. 81–85, 2016.
T. Sunartatie, “Trichophyton mentagrophytes sebagai Agen Penyebab Dermatofitosis pada Kambing,” J. sain Vet., vol. 28, no. 1, pp. 48–52, 2010.
C. K. Campbell, E. M. Johnson, and D. W. Warnock, Identification of Pathogenic Fungi, 2nd ed., vol. 59, no. 7. West Sussex: Wiley-Blackwell, 2013.
PDF Downloads: 208
How to Cite
Write scientific names with Italic fonts:
Copyright (c) 2022 Agung Bimantara, Hary Widjajanti, Elisa Nurnawati
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
d. Authors hold the copyright and retain publishing right of articles without restrictions.