• Budi Eko Wahyudi Department of Biosciences Program, Faculty of Science, Sriwijaya University, Padang Selasa Street 524, Palembang, South Sumatra 30139, Indonesia.
  • Salni Salni Department of Biology, Faculty of Mathematics & Natural Sciences, Sriwijaya University, Palembang-Prabumulih Highway km 32, Indralaya, Indonesia.
  • Arum Setiawan Department of Biology, Faculty of Mathematics & Natural Sciences, Sriwijaya University, Palembang-Prabumulih Highway km 32, Indralaya, Indonesia.

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tembesu , free radicals , antioxidant


Herbal medicine is becoming a trend in curing several diseases especially degenerative because of the bad effects of synthetic drugs. Tembesu plant (Fagraea fragrans Roxb.) is traditionally used for degenerative diseases. This study aims to separate bioactive compounds (active fraction), pure isolate compounds from tembesu leaf, then observe antioxidant activity. The methods used are maceration extraction, liquid-liquid fractionation, purification of compounds by gravity chromatography column and antioxidant activity by scavenging DPPH radicals. The results showed that the active antioxidant fractions were n-hexane and ethyl acetate fractions. The results of purification of antioxidant compounds, obtained 6 isolates N1, N2, N4, N5, E8 and E11. Antioxidant activity (IC50) of pure isolates N1 (13.72 ppm), N2 (28.93 ppm), N4 (110.44 ppm), N5 (177.23 ppm), E8 (82.50 ppm) and E11 (12 ,86 ppm). The antioxidant compounds that have been isolated are terpenes (N1, N2, N4, E8), phenols (N5) and flavonoids (E11). This study concluded that tembesu leaf have antioxidant potential. Antioxidant bioactive compounds in tembesu leaf have the potential to be developed into standardized herbs and phytopharmaceuticals.

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How to Cite
Write scientific names with Italic fonts:

Eko Wahyudi, B. ., Salni, S., & Setiawan, A. . (2022). ANTIOXIDANT ACTIVITY OF TEMBESU (Fagraea fragrans Roxb.) LEAF. BIOVALENTIA: Biological Research Journal, 8(2), 96–102.



Vol 8, No 2 (2022): Nov 2022

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