• Budi Eko Wahyudi Department of Biosciences Program, Faculty of Science, Sriwijaya University, Padang Selasa Street 524, Palembang, South Sumatra 30139, Indonesia.
  • Salni Department of Biology, Faculty of Mathematics & Natural Sciences, Sriwijaya University, Palembang-Prabumulih Highway km 32, Indralaya, Indonesia.
  • Arum Setiawan Department of Biology, Faculty of Mathematics & Natural Sciences, Sriwijaya University, Palembang-Prabumulih Highway km 32, Indralaya, Indonesia.

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tembesu , free radicals , antioxidant


Herbal medicine is becoming a trend in curing several diseases especially degenerative because of the bad effects of synthetic drugs. Tembesu plant (Fagraea fragrans Roxb.) is traditionally used for degenerative diseases. This study aims to separate bioactive compounds (active fraction), pure isolate compounds from tembesu leaf, then observe antioxidant activity. The methods used are maceration extraction, liquid-liquid fractionation, purification of compounds by gravity chromatography column and antioxidant activity by scavenging DPPH radicals. The results showed that the active antioxidant fractions were n-hexane and ethyl acetate fractions. The results of purification of antioxidant compounds, obtained 6 isolates N1, N2, N4, N5, E8 and E11. Antioxidant activity (IC50) of pure isolates N1 (13.72 ppm), N2 (28.93 ppm), N4 (110.44 ppm), N5 (177.23 ppm), E8 (82.50 ppm) and E11 (12 ,86 ppm). The antioxidant compounds that have been isolated are terpenes (N1, N2, N4, E8), phenols (N5) and flavonoids (E11). This study concluded that tembesu leaf have antioxidant potential. Antioxidant bioactive compounds in tembesu leaf have the potential to be developed into standardized herbs and phytopharmaceuticals.

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M. Valko, D. Leibfritz, J. Moncol, M. T. D. Cronin, M. Mazur, and J. Telser, “Free radicals and antioxidants in normal physiological functions and human disease,” Int. J. Biochem. Cell Biol., vol. 39, no. 1, pp. 44–84, 2007, doi: 10.1016/j.biocel.2006.07.001. DOI:

J. S. Johansen, A. K. Harris, D. J. Rychly, and A. Ergul, “Oxidative stress and the use of antioxidants in diabetes: Linking basic science to clinical pratice,” Cardiovasc. Diabetol., vol. 4, pp. 1–11, 2005, doi: 10.1186/1475-2840-4-5. DOI:

A. Phaniendra, D. B. Jestadi, and L. Periyasamy, “Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases,” Indian J. Clin. Biochem., vol. 30, no. 1, pp. 11–26, 2015, doi: 10.1007/s12291-014-0446-0. DOI:

L. A. Pham, H. He, and C. Pham, “Free Radicals, Antioxidants in Disease and Health Lien,” Int. J. Biomed. Sci., vol. 4, no. 2, 2008, doi: 10.17094/ataunivbd.483253.

B. W. Meier, J. D. Gomez, O. V. Kirichenko, and J. A. Thompson, “Mechanistic Basis for Inflammation and Tumor Promotion in Lungs of BHT-Treated Mice: Electrophilic Metabolites Alkylate and Inactivate Antioxidant Enzymes,” Chem Res Toxicol, vol. 20, no. 2, pp. 199–207, 2007, doi: 10.1021/tx060214f.Mechanistic. DOI:

O. Komalasari, S. Maryani, O. Juairiyah, and D. Novriadhy, “‘Tantangan dan Solusi Pengembangan PAJALE dan Kelapa Sawit Generasi Kedua (Replanting) di Lahan Suboptimal’ Kearifan Lokal Masyarakat Desa Bakung dalam memanfaatkan Resam (Gleichenia linearis), Seduduk (Melastoma malabathricum) dan Tembesu (Fagraea fragra,” no. June 2021, pp. 354–359, 2018.

I. M. C. Brighente, M. Dias, L. G. Verdi, and M. G. Pizzolatti, “Antioxidant activity and total phenolic content of some Brazilian species,” Pharm. Biol., vol. 45, no. 2, pp. 156–161, 2007, doi: 10.1080/13880200601113131. DOI:

N. E. Utami, S. Salni, and M. Verawaty, “Antioxidant activity leaves katimaha (Kleinhovia hospita L.),” Biovalentia, vol. 8, no. 1, pp. 22–31, 2022. DOI:

S. V. Duniya, M. C. Ojonugwa, A. D. Adamu, O. John, S. I. Eleojo, and U. O. Salifu, “Phytochemical constituent , percentage yield and phenolic content estimation of different solvent system of Caricapapaya leave article info International Journal of Chemistry and Phytochemical constituent , percentage yield and phenolic content e,” no. January, pp. 5–10, 2018.

M. O. Egua, E. U. Etuk, S. O. Bello, and S. W. Hassan, “Antidiabetic potential of liquid-liquid partition fractions of ethanolic seed extract of Corchorus olitorious,” J. Pharmacogn. Phyther., vol. 6, no. 1, pp. 4–9, 2014, doi: 10.5897/JPP2013.0294. DOI:

S. K. Algfri, M. Alshakka, and R. T. Munaiem, “International Journal of Pharmacy and Integrated Life Sciences Antioxidant Activity of The Leaves Of Acacia nilotica subspecies kraussiana,” Int. J. Pharm. Integr. Life Sci., vol. 3, no. 10, 2015.

D. C. Harris, Quantitative Chemical Analysis. New York: W.H. Freeman and Company, 2015.

M. Tegegne, E. Abiyu, S. Libesu, B. Bedemo, and M. Lewoyehu, “Phytochemical investigation, antioxidant and antibacterial activities of the fruit extracts of Solanum anguivi,” Biotechnol. Biotechnol. Equip., vol. 35, no. 1, pp. 1480–1491, 2021, doi: 10.1080/13102818.2021.1993087. DOI:

P. Kumara, S. K, and A. Kumar B, “Determination of DPPH Free Radical Scavenging Activity by RP-HPLC, Rapid Sensitive Method for the Screening of Berry Fruit Juice Freeze Dried Extract,” Nat. Prod. Chem. Res., vol. 06, no. 05, 2018, doi: 10.4172/2329-6836.1000341. DOI:

D. H. Truong, D. H. Nguyen, N. T. A. Ta, A. V. Bui, T. H. Do, and H. C. Nguyen, “Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro anti-inflammatory activities of severinia buxifolia,” J. Food Qual., vol. 2019, 2019, doi: 10.1155/2019/8178294. DOI:

J. Omale, “Cytotoxicity and Antioxidant Screening of Some Selected Nigerian Medicinal Plants,” Asian J. Pharm. Clin. Res., vol. 2, no. 4, 2021.

A. A. Bele and A. Khale, “An Overview On Thin Layer Chromatography H. K. College of Pharmacy, Jogeshwari (W), Mumbai, Maharashtra, India,” Int. J. Pharm. Sci. Res., vol. 2, no. 2, pp. 256–267, 2011.

P. C. Eklund, O. K. Langvik, J. P. Warna, T. O. Salmi, S. M. Willfor, and R. E. Sjoholm, “Chemical studies on antioxidant mechanisms and free radical scavenging properties of lignans,” Org. Biomol. Chem., vol. 3, pp. 3336–3347, 2005. DOI:

P. M. Arce, F. A. Beltrán, G. A. Manríquez, M. E. Cota, A. Quian, and R. G. Peralta, “Nutritional value of conventional, wild and organically produced fruits and vegetables available in Baja California Sur markets,” Terra Latinoam., vol. 37, no. 4, pp. 401–406, 2019, doi: 10.28940/terra.v37i4.524. DOI:

M. Gangwar, M. K. Gautam, A. K. Sharma, Y. B. Tripathi, R. K. Goel, and G. Nath, “Antioxidant Capacity and Radical Scavenging Effect of Polyphenol Rich Mallotus philippenensis Fruit Extract on Human Erythrocytes : An In Vitro Study,” vol. 2014, 2014. DOI:

R. Baharfar, R. Azimi, and M. Mohseni, “Antioxidant and antibacterial activity of flavonoid- , polyphenol- and anthocyanin-rich extracts from Thymus kotschyanus boiss & hohen aerial parts,” vol. 52, no. October, pp. 6777–6783, 2015, doi: 10.1007/s13197-015-1752-0. DOI:

M. Shahriar, M. Haque Aminul, and S. Islam Ashraful, “Antimicrobial , Cytotoxicity and Antioxidant Activity of Tinospora crispa,” J. Pharm. Biomed. Sci., vol. 13, no. 12, pp. 1–4, 2011.

S. Phongpaichit et al., “Biological activities of extracts from endophytic fungi isolated from Garcinia plants,” FEMS Immunol. Med. Microbiol., vol. 51, no. 3, pp. 517–525, 2007, doi: 10.1111/j.1574-695X.2007.00331.x. DOI:

R. T. Martínez et al., “Antioxidant activity of the essential oil and its major terpenes of Satureja macrostema (Moc. and Sessé ex Benth.) Briq.,” Pharmacogn. Mag., vol. 13, no. 52, pp. S875–S880, 2017, doi: 10.4103/

M. P. Gomez and E. Gonzalez, “Terpene Compounds in Nature: A Review of Their Potential Antioxidant Activity,” Curr. Med. Chem., vol. 19, no. 31, pp. 5319–5341, 2012, doi: 10.2174/092986712803833335. DOI:

C. Y. Wang, Y. W. Chen, and C. Y. Hou, “Antioxidant and antibacterial activity of seven predominant terpenoids,” Int. J. Food Prop., vol. 22, no. 1, pp. 230–238, 2019, doi: 10.1080/10942912.2019.1582541. DOI:

H. Zhang and R. Tsao, “Dietary polyphenols, oxidative stress and antioxidant and anti-inflammatory effects,” Curr. Opin. Food Sci., vol. 8, pp. 33–42, 2016, doi: 10.1016/j.cofs.2016.02.002. DOI:

A. Zeb, “Concept, mechanism, and applications of phenolic antioxidants in foods,” J. Food Biochem., vol. 44, no. 9, pp. 1–22, 2020, doi: 10.1111/jfbc.13394. DOI:

M. C. Foti, “Antioxidant properties of phenols,” J. Pharm. Pharmacol., vol. 59, no. 12, pp. 1673–1685, 2007, doi: 10.1211/jpp.59.12.0010. DOI:

A. N. Panche, A. D. Diwan, and S. R. Chandra, “Flavonoids: An overview,” J. Nutr. Sci., vol. 5, 2016, doi: 10.1017/jns.2016.41. DOI:

G. B. Bubols et al., “Send Orders of Reprints at The Antioxidant Activity of Coumarins and Flavonoids,” Rev. Med. Chem., vol. 13, pp. 318–334, 2013. DOI:

M. R. Santos and L. Mira, “Protection by flavonoids against the peroxynitrite-mediated oxidation of dihydrorhodamine,” Free Radic. Res., vol. 38, no. 9, pp. 1011–1018, 2004, doi: 10.1080/10715760400003384. DOI:

M. Moalin et al., “A planar conformation and the hydroxyl groups in the B and C rings play a pivotal role in the antioxidant capacity of quercetin and quercetin derivatives,” Molecules, vol. 16, no. 11, pp. 9636–9650, 2011, doi: 10.3390/molecules161196. DOI:



How to Cite
Write scientific names with Italic fonts:

Eko Wahyudi, B. ., Salni, S., & Setiawan, A. . (2022). ANTIOXIDANT ACTIVITY OF TEMBESU (Fagraea fragrans Roxb.) LEAF. BIOVALENTIA: Biological Research Journal, 8(2), 96–102.



Vol 8, No 2 (2022): Nov 2022

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