QUANTITATIVE AND QUALITATIVE TEST OF THE FECAL SAMPLE FROM SUMATRAN ELEPHANT (Elephas maximus sumatranus)
DOI:
https://doi.org/10.24233/biov.6.2.2020.163Keywords:
Fecal DNA , Padang Sugihan Landscape , South Sumatra , Sumatran ElephantAbstract
Sumatran Elephants (Elephas maximus sumatranus) is one of the endemic Sumatran fauna that is protected by Indonesia Law. The Remnant Forest of Acacia District Penyabungan in the landscape of Padang Sugihan is one of the elephant’s habitats in South Sumatra. Habitat degradation and fragmentation, poaching and conflicts become the major threats for the elephant population. Genetic study is one of the efforts to establish a conservation policy. It is necessary to test the quantitative and qualitative of DNA, at the extractions stage from fecal samples, in order to get the standard results on DNA concentration which will be analyzed later. The method used is through direct observation as the initial survey to determine the sampling location. Fecal samples were preserved in absolute ethanol at -20°C. The extraction process was carried out using the Quick-DNATM Fecal/Soil Microbe Miniprep Kit. Samples were tested quantitatively using a Nanodrop Thermo Scientific, with the expectation that the concentration results were considered to be 1.6-1.8 and continued with the qualitative test of DNA through electrophoresis gel agarose and UV Transilluminator. Of the 20 samples, only 12 samples showed positive results (there were DNA bands). The results of this quantitative and qualitative test could be used to determine a viable sample to be used as a product in the genetic analysis stage of Sumatran Elephants in the Remnant Forest of Acacia South Sumatra.
Last Year PDF Downloads
References
Bourgeois S, Kaden J, Senn H, Bunnefeld N, Jeffery KJ, Akomo-Okoue EF, Ogden R, and McEwing. 2019. Improving cost-effeciency of faecal genotyping: New Tools foe elephant species. Htpps://doi.org/10.1371/journal.pone0210811.1-16.
Choudhury A, Lahiri Choudhury DK, Desai, Duckworth JW, Easa PS, Johnsingh AJT, Fernando P, Hedges S, Gunawardena M, Kurt, F, Karanth U, Lister A, Menon V, Riddle H, Rübel A, and Wikramanayake E. 2008. IUCN SSC Asian Elephant Specialist Group Elephas maximus. The IUCN Red List of Threatened Species 2008: e.T7140A12828813. DOI: 10.2305/IUCN.UK.2008.RLTS.T7140A12828813.en.
Devereux R, and Wilkinson SS. 2004. Amplification of ribosomal RNA sequences. Molecular Microbial Ecology Manual, Second Edition 3: 509–522.
Faatih M. 2009. Isolasi dan Digesti DNA Kromosom. Jurnal Penelitian Sains dan Teknologi 1: 61-67.
Fatchiyah, A.E.L., Widyarti, S., dan Rahayu, S. 2011. Biologi Molekuler: Prinsip Dasar Analisis. Jakarta: Erlangga. xxiv + 191hlm.
Fleischer RC, Perry EA, Muralidharan K, Stevens EE, and Wemmer CM. 2001. Phylogeography of the Asian elephant (Elephas maximus) based on mitochondrial DNA. Evolution 55: 1882–1892.
Fernando, P., Frender, M.E., Encalada, S.E., & Lande, R. 2000. Mitochondrial DNA Variation, Phyleogeography and Population Structure of the Asian Elephant. Heredity. 84: 362-72.
Fernando, P., Vidya , TNC., Rajapakse, C., Dangolla, A., & Melnick. DJ. 2003. Reliable non-invasive genotyping: Fantasy or reality? J Hered 94: 115–123.
Gopala A, Hadian O, Sunarto, Sitompul A, Williams A, Leimgruber P, Chambliss SE, and Gunaryadi D. 2011. Elephas maximus ssp. sumatranus. The IUCN Red List of Threatened Species. http://dx.doi.org/10.2305/IUCN.UK2011-2.RLTS.T199856A9129626.en. Downloaded on 05 October 2018.
Peraturan Pemerintah Nomor 7 Tahun 1999 tentang Pengawetan Jenis Tumbuhan dan Satwa.
Peraturan Menteri Lingkungan Hidup dan Kehutanan Republik Indonesia Nomor P.106/ MENLHK/SETJEN/KUM.1
/12/2018 Tentang Perubahan Kedua Atas Peraturan Menteri Lingkungan Hidup dan Kehutanan Republik Indonesia Nomor P.20/ MENLHK/SETJEN/KUM.1/6/2018 Tentang Jenis Tumbuhan dan Satwa Yang Dilindungi.
Soehartono T, Susilo HD, Sitompul AF, Gunaryadi D, Purastuti EM, Azmi W, Fadhli N, dan Stremme C. 2007. Strategi dan Rencana Aksi Konservasi Gajah Sumatera dan Gajah Kalimantan 2007-2017. Jakarta.
Sulandari S, dan Zein MSA. 2012. Mitochondrial DNA Variation of The Sumatran Elephant Populations In Sumatera, Indonesia. Biotropia. 19: 92-102.
Susilowati O, Mahanani AI, Yustian I, Setiawan D, dan Sumantri H. 2016. Identifikasi dan Pemetaan Kantong-Kantong Habitat Gajah dan Harimau di Sumatera Selatan. FMIPA Universitas Sriwijaya, Indralaya. xiii+64 hlmn.
Syahri BF, Gunawan H, dan Sudoyo H. 2015. Analisis Mikrosatelit Pada Sampel Feses Gajah Sumatera (Elephas maximus sumatranus) di Taman Nasional Tesso Nilo, Riau. Jurnal JOM FMIPA 2: 42-49.
Zein, M.S.A, dan Dewi, P.M. 2013. DNA Barcode Fauna Indonesia. Jakarta; Kencana-Prenadamedia Grup. Xx + 242 hlm.
Zein MSA, dan Sulandari S. 2015. Kajian gen Amely Gajah Sumatra (Elephas maximus sumatranus) [The Study of Amely Gen of Sumatran elephant (Elephas maximus sumatranus)]. Jurnal Biologi Indonesia 12: 81-86.
ZYMO REASERCH FECAL/MIKROBE KIT. HTTPS://WWW.ZYMORESEARCH.COM/COLLECTIONS/QUICK-DNA-FECAL-SOIL-MICROBE-KITS.
PDF Downloads: 365
Published
How to Cite
Write scientific names with Italic fonts:
Issue
Section
Copyright (c) 2020 Winda Indriati, Indra Yustian, Arum Setiawan
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
d. Authors hold the copyright and retain publishing right of articles without restrictions.
.gif)
