• Yulia Pujiastuti Plant Protection Department, Faculty of Agriculture, Sriwijaya University, South Sumatera, Indonesia
  • M Ridwan Plant Protection Department, Faculty of Agriculture, Sriwijaya University, South Sumatera, Indonesia
  • H Saputra Plant Protection Department, Faculty of Agriculture, Sriwijaya University, South Sumatera, Indonesia
  • M Agusrafil Plant Protection Department, Faculty of Agriculture, Sriwijaya University, South Sumatera, Indonesia
  • A Nurnabila Plant Protection Department, Faculty of Agriculture, Sriwijaya University, South Sumatera, Indonesia
  • Henny Hendarjanti PT. Astra Agro Lestari. Kawasan Industri Pulo Gadung, Blok OR 1-2, Jatinegara, Jakarta Timur 13920, Jakarta, Indonesia

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Bacillus thuringiensis , Bio-insecticide , oil palm , mortality , Oryctes rhinoceros


Oryctes rhinoceros is an important pest in oil palm plantations. Adult stage of the beetle causes damage, however larval stage is very important to be controlled to break the cycle of life. An entomopathogenic bacterium Bacillus thuringiensis is one of biological agents to control these insects. Its toxic protein content provides specific insect targets as stomach poison. Objectives  of the research was to study the impact of B. thuringiensis propagated in bio-urine enriched with 5 per cent molasses towards  Oryctes larvae. The research was carried out in the shade house of Plant Protection Study Program, Faculty of Agriculture, Sriwijaya University from August to November 2021. Experiment was designed in a randomized complete block design with 7 treatments and 4 replications. A total of 20 ml of bio-insecticide was dissolved in 280 ml of water, sprayed evenly on the soil mixed with  male palm flowers as feed of larvae. The treatments were 6 isolates of B. thuringiensis isolated from soil in oilpalm plantation, namely with codes:  C14, C15, A15, OJ, BK, and LK as well. The results showed density of B. thuringiensis spores in bio-urine media was different in each isolate. The highest spore density in isolate code LK was 4.83 x 1010 spores/ml and the lowest (in isolate A15) was at 3.5 x 1010 spores/ml. Mortality rates were significantly different between isolate treatments starting from day 3 to day 12 of observation. C15 isolate lead the highest mortality rate of 100% on day 12 while other isolates showed mortality data below 100% (88-98%). Body weight and length showed significantly differences on days 0, 6 and 12 after application. Symptoms of infection begin with a change in skin color from white to brown, dark brown and black. Death is characterized by a soft body texture and wet rot.

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Bravo,A. Isabel Gómez, Helena Porta, Blanca Ines García-Gómez, Claudia Rodriguez-Almazan, Liliana Pardo, and Mario So-berón..2013. Evolution Of Bacillus thurin-giensis Cry Toxins Insecticidal Activity. Microb Biotechnol. 2013 Jan; 6(1): 17–26. Doi: 10.1111/J.1751-7915.2012.00342.X Pmcid: Pmc3815381 DOI:

Magallon J.E., V.M Hernandez-Velazquez , A.A.Alvear-Garcia , I. Arenas-Sosa, G. Peña-Chora. 2015. Evaluation of industrial by-products for the production of Bacillus thuringiensis strain GP139 and the pathogenicity when applied to Bemisia tabaci nymphs. Bulletin of Insectology 68 (1): 103-109, 2015 ISSN 1721-8861

Poopathi, S, Mani, C. and Rajeswari, G. 2013. Po-tential of sugarcane bagasse (agro-industrial waste) for the production of Bacillus thuringiensis is-raelensis. Tropical Biomedicine 30(3): 504–515 (2013)

Zou H.,Shicun Ding ,Wenyu Zhanga, Juejun Yaoa,Lin Jiang ,Jing Liang. 2016. Study on influ-ence factors in Bacillus thuringiensis production by semi-solid state fermentation usingfoodwaste The Tenth International Conference on Waste Management and Technology (ICWMT) Procedia Environmental Sciences 31 ( 2016 ) 127 – 135 DOI:

Valicente F.H., Edmar De S.T., Maria I.S.L., Fer-nanda, L.F. and Corina M.V. 2010. Production of Bacillus thuringiensis Biopesticide Using Commercial Lab Medium And Agricultural By-Products As Nutrient Sources. Revista Brasileira De Milho E Sorgo, V.9, N.1, P. 1-11, 2010 DOI:

Wahyuono, D. 2015. Kajian Formulasi Bacillus thuringiensis Dengan Carrier Limbah Cair Pabrik Kelapa Sawit Untuk Pengendalian Ulat Api (Setora nitens).Planta Tropika Journal of Agro Science Vol 3 No 1 / Februari 2015. DOI 10.18196/pt.2015.036.24-30. DOI:

Hosang dan Salim, 2013 Hosang, M. L. A., & Sal-im. 2013. Penekanan Populasi Oryctes rhinoceros dan Rhynchophorus ferrugineus dengan Perangkap dan Feromon. Balai Penelitian Tana-man Palma Manado, 65–72

Priwiratama,H., A.E.Prasetyo, T.A.P.Rozziansha, dan A.Susanto. 2020. Hama kumbang badak Oryctes rhinoceros Bioekologi, kerusakan dan pengendalian. Pusat Penelitian Kelapa sawit Me-dan. 51 hal.

Susanto, A, Sudharto, dan AE Prasetyo. 2011. In-formasi Organisme Pengganggu Tanaman Kumbang Tanduk Oryctes rhinoceros Linn.Artikel. Pusat Penelitian Kelapa Sawit: Me-dan.

Pujiastuti,Y. Janri Setiawan G. dan Arinafril. 2010. Pendugaan Perkembangan Populasi Oryctes rhinoceros L. (Coleoptera: Scarabaeidae) Di Perkebunan Kelapa Sawit (Elaeis Guineensis Jacq.) JRL. Vol. 6 No. 2, Juli 2010 : 199 – 205 DOI:

Pujiastuti,,Y. A.Arsi, S. Sandi. 2020. Character-istics of Bacillus thuringiensis isolates indigenous soil of South Sumatra (indoneisa) and their patho-genicity against oil palm pests Oryctes rhinoceros (Coleoptrea:Scarabaeidae). Biodiversitas Vol 21 No.4 April 2020, PP 1287-1294 DOI:

Bravo, A., Supaporn Likitvivatanavong, Sarjeet S. Gillb, and Mario Soberóna. 2011. Bacillus thu-ringiensis: A Story of A Successful Bioinsecticide. Insect Biochem Mol Biol. 2011 July ; 41(7): 423–431. DOI:

Kamaruddin, N., Z.A. Isa, M. Abdullah dan MB Wahid. 2001. Factors affecting development of Oryctes rhinoceros in some substrates com-monly found in the oil palm environment. Journal of Oil Palm Research 13((1)):64-74

Lacey LA, Grzywacz D, Shapiro-Ilan DI, Fru-tos R, Brownbridge M, Goettel MS. 2015. Insect pathogens as biological control agents: Back to the future. J Invertebr Pathol. 2015 Nov; 132:1-41. DOI:

Palma L., Delia Muñoz , Colin Berry, Jesús Murillo and Primitivo Caballero. 2014. Bacillus thuringiensis Toxins: An Overview of Their Bio-cidal Activity. Toxins. Vol.6: 3296-3325; doi:10.3390/toxins 6123296 toxins ISSN 2072-6651 /journal/toxins DOI:

Zalunin, I.A. & Elpidina, Elena & Oppert, Brenda. 2015. The role of proteolysis in the bio-logical activity of Bt insecticidal crystal proteins. Bt Resistance: Characterization and Strategies for GM Crops Producing Bacillus thuringiensis Tox-ins. (eds M. Soberón, Y. Gao and A. Bravo) 107-118. DOI:

Schünemann, Rogério & Knaak, Neiva & Fiu-za, Lidia. (2014). Mode of Action and Specificity of Bacillus thuringiensis Toxins in the Control of Caterpillars and Stink Bugs in Soybean Culture. ISRN microbiology. 2014. 135675. 10.1155/2014/135675. DOI:

Zhang Y., Yan Ma, Pin-Jun Wan, Li-Li Mu, and Guo-Qing Li. 2013. Bacillus thurin-giensis Insecticidal Crystal Proteins Affect Lifespan and Reproductive Performance of Helicoverpa armigera and Spodoptera ex-igua Adults. Journal of Economic Entomology 106(2):614-621. 2013 DOI:

Nuriyanti DD, Widhiono I, Suyanto A. 2016. Faktorfaktor ekologis yang berpengaruh terhadap struktur populasi kumbang badak (Oryctes rhi-noceros L.). Biosfera 33:13–21. doi: https:// DOI:



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Pujiastuti, Y., Ridwan, M., Saputra, H., Agusrafil, M., Nurnabila, A., & Hendarjanti, H. . (2022). STUDY OF EFFECTIVITY Bacillus thuringiensis BASED BIO-INSECTICIDE AGAINST Oryctes rhinoceros LARVAE AT SHADE HOUSE. BIOVALENTIA: Biological Research Journal, 8(2), 145–150.



Vol 8, No 2 (2022): Nov 2022