ANTIOXIDANT ACTIVITY LEAVES KATIMAHA (Kleinhovia hospita L.)
Keywords:Katimaha , Antioxidant , DPPH
The free radicals that people face in their daily activities require humans to get additional antioxidants from the outside. To overcome this problem, it is necessary to look for many sources of natural antioxidants considering the need for antioxidants that are increasingly needed Katimaha (K. hospita L.) is believed to have many benefits in the field of medicine. Therefore, katimaha has the potential to be used as a source of natural antioxidants. The research methods carried out were refining katimaha leaf simplicia, extraction, fractionation, antioxidant activity test with TLC plate, purification, determination of compound class, and antioxidant activity test using the DPPH method. The results showed that the active fraction of Katimaha leaf was the n-hexane fraction and the ethyl acetate fraction. Column chromatography on the active fraction found six pure eluates that had antioxidant activity, namely N1, N3, N5, E1, E2, and E4. Groups of compounds N1, N3, N5, E1 are terpenoids, group E2 eluates are tannins, while E3 eluates are flavonoid compounds. The pure eluate antioxidant activity test using the DPPH method obtained IC50 values of N1 are 40,142 ppm, N3 80,057 ppm, N5 271,643 ppm and E1 89,16 ppm, E2 124.91 ppm and E4 52.51 ppm.
Last Year PDF Downloads
Dini I and Darminto, “A method of isolation bioaktif katimaha compound in plants,” Journal Chemica, Vol.13, No. 2, PP. 11–16. 2012.
E.R. Yuslianti, Introductory free radicals and antioxidant. Yogyakarta: Budi utomo, 2018.
N. Rahmawati, N.H. Prayoga, and M. Rahmah, “Isolation and the antioxidant activity from the compound of a metabolite secondary n-butanol fig (Ficus carica L.) leaves Varietas Brown Turkey. Journal Penelitian Farmasi Indonesia,” Vol. 8, No. 1, PP.24-30. 2019.
W. F. Dewatisari, L. Rumiyanti, and I. Rahmawati, 2017, “Rendemen and screening phytochemical on extract leaves Sanseviera sp. Agricultural research journal applied,” Vol.17, No.3, PP. 197- 201. 2017.
R. Uthia, H. Arifin, and F. Efrianti, “The influence of the fractionate extract basil leaves (Ocinum sanctum L.) on the activities of the arrangement of the nerves center on mice white male,” Journal Farmasi Hidea. Vol. 9, No.1, PP. 1-7. 2017.
D. E. Oktaviantari, N. Feladita, and R, Agustin, “Identification hydroquinone in soap bleach facial cleansers on three clinics beauty in bandar lampung with the thin and spekrofotometri uv-vis layer chromatograph,” Jurnal Analisi farmasi. Vol. 4, No.2, PP. 91-97. 2019.
D. Sukandar, H. Sandra, and A. Imamah, “The activity of a compound antidiabetic drug ektrak ethyl acetate leaves of the pandanus fragrant (Pandanus Amaryllifolius Roxb.)” Journal Valensi. Vol. 1, No. 6, PP. 269-273. 2010.
N. F. N. Wati, “Improving the quality of both the patchouli use through a adsorption adsorbent alumina with flow system,” Indonesian Journal of Chemical Research. Vol. 1, No. 2, PP. 84-95. 2014.
Salni, H. Marisa, and W, Mukti, “Isolation of leaves jengkol (Pithecolobium lobatum Benth) antibacterial compounds and the determination of the KHM. Journal Penelitian Sains,” Vol. 14, No. 1, PP. 1-4. 2011.
V, Handayani. A.R, Ahmad and M, Sudir, “The antioxidant activity extract methanol patikala (Etlingera elatior (Jack) R.M.Sm) flowers and leaves Uses the method DPPH,” Journal Pharmacy Science Research, Vol. 1, No. 2, PP.86-93. 2014.
D. Purwanto, B. Syaiful and R. Ahmad, “The antioxidant activity purnajiwa (Kopsia Arborea Blume.) fruit extract with a solvent,” Journal Riset Kimia. Vol. 3, No. 1, PP. 34-32. 2017.
N. N. Yuliani, J. Sambara, and M.A. Mau, “The antioxidant activity ethyl acetate faction be extract ethanol rhizomes ginger red (Zingiber officinale var Rubrum) with thw methods DPPH (1,1-Difenil-2- Pikrilhidrazil). Journal Info Kesehatan. Vol. 14, No. 1, PP. 1-21. 2016.
N. C. Suryani, D. G. M. Permana, and A. A. G. N. A. Jambe, “The influence of a solvent to the total flavonoid antioxidant activity and extract matoa (Pometia pinnata) leaves. Food journal knowledge and technology udayana,” Vol. 1, No.2, PP. 1-10. 2015.
J. Omale, and P.O. Nnachieta, “Cytotoxicity and Antioxidant Screening of Some Selected Nigerian Medicinal Plants,” Asian Journal of Pharmaceutical and Clinical Research, Vol. 2, No. 4, PP. 48-53. 2009.
S. P. S. Dia, Nurjanah, and M.A. Jacoeb, “The chemical composition and antioxidant activity, roots the skin lindur stems and leaves,” Jurnal JPHPI, Vol. 18, No. 2, PP. 205-219. 2015.
B.Sopiah, H. Muliasari, and E. Yuanita, “Screening phytochemistry and potential antioxidant activity extract ethanol evergreen leaves and red leaves euphorbia pulcherrima,” Journal Ilmu Kefarmasian Indonesia, Vol. 17, No.1, PP. 27-33. 2019.
K. Khaira, “Preventing free radical with antioxidant,” Journal Sainstek. Vol. 2, No. 2, PP. 183-187. 2010.
F. Firdiyani, W.T. Agustini, and F.W. Ma’ruf, “The extraction of natural as antioxidant compounds bioaktif spirulina platensis fresh different with a solvent. Journal JPHPI, Vol. 18, No.1, PP. 28-37. 2015.
V. Tanaya, R. Retnowati, and Suratno, “The spring polar from the leaves of Mangga Kasturi (Mangifera casturi Kosterm). Journal Kimia Student, Vol. 1, No.1, PP. 778-784. 2015.
R.M. Podungge, K.Y. Salimi, and S. Duengo, “Isolasi dan Uji Aktivitas Antioksidan Senyawa Flavonoid dari Daun Miana Isolation and the flavonoid antioxidant activity compound miana (Coleus Scutelleroides Benth.) of leaves. Journal Entropi, Vol. 12, No. 1, PP. 64-74. 2017.
D. S. Hardiningtyas, S. Purwaningsih, and E. Handharyani, “Antioxidant activity and effects hepatoprotektif leaves mangroves api-api white,” Journal JPHPI, Vol. 17, No.1, PP. 80-91. 2014.
P. Paraeng, H. D. Mantiri, and A. Rumengan, “The antioxidant activity in macro brown algae hydroclathrus clathratus (C.AGARDH) hower Padina and minor Yamada. Journal Pesisir dan Laut Tropis, Vol. 2, No.1,PP. 37-43. 2016.
R. N. Fathurrahman, and I. Musfiroh, “Analysis technique instrumentation tannin compounds,” Journal Farmaka, Vol. 16, No.2, PP.449-456. 2018.
E. O. Puspa, I. Syahbanu, and A.M. Wibowo, “The phytochemistry toxicity and volatile oil leaves nutmeg (Myristica fragans houtt) lemukutan of the island,” Journal JKK, Vol. 6, No.2, PP. 1-6, 2017.
Yuhernita and Juniarti, “A compound of a metabolite secondary analysis of an extract methanol surian leaves which have the potential to as antioxidant,” Journal Makara Sains, Vol. 15, No.1, PP. 48-52. 2011.
P. Molyneux, “The Use of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH), For Estimating Antioxidant Activity,” Songklanakarin J. Sci. Technol, Vol. 26, No.2, PP. 211-219. 2004.
S. Maryam, M. Baits, and A. Nadia, “The antioxidant activity extract ethanol leaves kelor (Moringa oleifera Lam.) using that method FRAP (Ferric Reducing Antioxidant Power), Journal Fitofarmaka Indonesia Vol. 2, No.2, PP. 115-118. 2015.
D. Purwanto, B. Syaiful, and R. Ahmad, “The antioxidant activity purnajiwa (Kopsia arborea Blume.) fruit extract with a solvent,” Journal Riset Kimia, Vol. 3, No.1, PP. 34-32, 2017.
How to Cite
Write scientific names with Italic fonts:
Copyright (c) 2021 Authors
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a License Creative Commons Attribution-ShareAlike 4.0 International that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
d. Authors hold the copyright and retain publishing right of articles without restrictions.