SELECTION OF ANTAGONISTIC RHIZOBACTERIA POTENTIAL FOR BIOLOGICAL CONTROL OF Fusarium oxysporum
DOI:
https://doi.org/10.24233/biov.9.2.2023.416Keywords:
Wilt Disease , Inhibition test , fungicide , rhizosphere bacteriaAbstract
Fusarium oxysporum is a pathogen that causes wilt disease in many plants and can couse losses of up to fifty percent. Standard control is to use synthetic fungicides. However, continuous use of fungicides has a high environmental risk, so environmentally friendly and safe control efforts are needed. One way is by using rhizosphere bacteria or rhizobacteria. This research aims to obtain rhizobacteria isolates that can control the pathogen F. oxysporum, which causes fusarium wilt in vitro. This research was conducted from June to July 2023. The methods in this research included isolating rhizobacteria, observing the morphology of bacterial colonies, gram staining, gram reaction test, catalyst test, and in vitro antagonist test. Research data shows that of the thirty rhizobacteria isolates observed, all isolates had the potential to inhibit the growth of F. oxysporum. The KMTK2 showed the highest inhibitory ability isolate with an inhibitory ability of 73,99%, and the lowest inhibitory ability was shown by the TBA1 isolate with an inhibitory ability of 51,56%. Potential rhizobacteria isolates can suppress the growth of the pathogen F. oxysporum and can be used as a biological agent to reduce the use of pesticides in treating plant diseases.
Last Year PDF Downloads
References
S. N. Rampersad, “Pathogenomics and Management of Fusarium Diseases in Plants,” Pathogens, vol. 9, no. 340, pp. 1–21, 2020, doi: 10.3390/pathogens9050340. DOI: https://doi.org/10.3390/pathogens9050340
S. Soleha, R. Pratama, A. Muslim, S. Suwandi, and S. Kadir, “The Identification and Pathogenicity of Fusarium oxysporum Causing Acacia Seedling Wilt Disease,” J. For. Res., vol. 33, no. 2, pp. 711–719, 2022, doi: 10.1007/s11676-021-01355-3. DOI: https://doi.org/10.1007/s11676-021-01355-3
P. Fan et al., “Crop Rotation Suppresses Soil-borne Fusarium Wilt of Banana and Alters Microbial Communities,” Arch. Agron. Soil Sci., pp. 1–34, 2020, doi: 10.1080/03650340.2020.1839058. DOI: https://doi.org/10.1080/03650340.2020.1839058
A. Nur Abdila and M. Maduratna, “Uji Efektivitas Fungisida Nabati (Kombinasi Tepung Jagung Dan Ekstrak Daun Sirsak) Dalam Mengendalikan Penyakit Layu Fusarium (Fusarium Oxysporum) Pada Tanaman Cabai,” J. Nas. Holist. Sci., vol. 1, no. 1, pp. 17–20, 2021, doi: 10.56495/hs.v1i1.17. DOI: https://doi.org/10.56495/hs.v1i1.17
S. Carmona-Hernandez, J. J. Reyes-Pérez, R. G. Chiquito-Contreras, G. Rincon-Enriquez, C. R. Cerdan-Cabrera, and L. G. Hernandez-Montiel, “Biocontrol of postharvest fruit fungal diseases by bacterial antagonists: A review,” Agronomy, vol. 9, no. 121, pp. 1–15, 2019, doi: 10.3390/agronomy9030121. DOI: https://doi.org/10.3390/agronomy9030121
A. Jamil, N. Musheer, and M. Kumar, “Evaluation of Biocontrol Agents for Management of Wilt Disease of Tomato Incited by Fusarium oxysporum f. sp. lycopersici,” Arch. Phytopathol. Plant Prot., vol. 54, no. 19–20, pp. 1722–1737, 2021, doi: 10.1080/03235408.2021.1938353. DOI: https://doi.org/10.1080/03235408.2021.1938353
M. M. R. Khalil et al., “Rhizospheric bacteria as potential biocontrol agents against Fusarium wilt and crown and root rot diseases in tomato,” Saudi J. Biol. Sci., vol. 28, no. 12, pp. 7460–7471, 2021, doi: 10.1016/j.sjbs.2021.08.043. DOI: https://doi.org/10.1016/j.sjbs.2021.08.043
D. Miljaković, J. Marinković, and S. Balešević-Tubić, “The Significance of Bacillus spp. In Disease Suppression and Growth Promotion of Field and Vegetable Crops,” Microorganisms, vol. 8, no. 1037, pp. 1–19, 2020, doi: 10.3390/microorganisms8071037. DOI: https://doi.org/10.3390/microorganisms8071037
M. T. El-Saadony et al., “Plant growth-promoting microorganisms as biocontrol agents of plant diseases: Mechanisms, challenges and future perspectives,” Front. Plant Sci., vol. 13, no. October, pp. 1–19, 2022, doi: 10.3389/fpls.2022.923880. DOI: https://doi.org/10.3389/fpls.2022.923880
L. Zhu, J. Huang, X. Lu, and C. Zhou, “Development of Plant Systemic Resistance By Beneficial Rhizobacteria : Recognition , Initiation , Elicitation and Regulation,” Front. Plant Sci., pp. 1–16, 2022, doi: 10.3389/fpls.2022.952397. DOI: https://doi.org/10.3389/fpls.2022.952397
Harlis, R. S. Budiarti, H. Kapli, and M. E. Sanjaya, “Produksi Pupuk Cair dari Isolat Bakteri Limbah Sayur Pasar Angso Duo Jambi dalam Meningkatkan Perekonomian dan Kesehatan Lingkungan Masyarakat Jambi,” Biospecies, vol. 12, no. 1, pp. 40–48, 2019, doi: 10.22437/biospecies.v12i1.6577. DOI: https://doi.org/10.22437/biospecies.v12i1.6577
A. I. R. Dalimunthe, Susanna, and L. Hakim, “Eksplorasi dan Karakterisasi Bakteri Endofit Asal Tanaman Padi Sawah di Kabupaten Aceh Besar,” J. Ilm. Mhs. Pertan., vol. 8, no. 3, pp. 550–564, 2023.
F. Lailatus Sa, N. Rahmadhini, and Suharto, “Eksplorasi dan Identifikasi Bacillus sp. dari Tanah Rizosfer Bambu dan Tomat di Kelurahan Made, Sambikerep, Surabaya,” Agrocentrum, vol. 1, no. 1, pp. 1–6, 2023. DOI: https://doi.org/10.33005/agrocentrum.v1i1.7
V. N. Tylova, S. Bahri, B. R. Juanda, and A. P. J. Kusdiana, “Potensi Bakteri Endofit sebagai Pengendali Biologis Cendawan Pestalotiopsis sp. Penyebab Penyakit Gugur Daun pada Tanaman Karet (Hevea brasiliensis Muell. Arg.),” J. Ilmu-Ilmu Pertan. Indones., vol. 25, no. 1, pp. 51–58, 2023, doi: 10.31186/jipi.25.1.51-58. DOI: https://doi.org/10.31186/jipi.25.1.51-58
R. Subula, W. D. Uno, and A. Abdul, “Kajian Tentang Kualitas Kompos Yang Menggunakan Bioaktivator Em4 (Effective Microorganism) Dan Mol (Mikroorganisme Lokal) Dari Keong Mas,” Jambura Edu Biosf. J., vol. 4, no. 2, pp. 54–64, 2022, doi: 10.34312/jebj.v4i2.7753. DOI: https://doi.org/10.34312/jebj.v4i2.7753
Paisal, E. Triwahyu, and H. Nirwanto, “Eksplorasi BakteriI Bacillus spp. pada Perakaran Tanaman Kentang (Solanum tuberosum L.) sebagai Agensia Pengendali Hayati Patogen Fusarium sp. Asal Lahan Wonokitri Kabupaten Pasuruan Jawa Timur,” J. Pertan. Agros, vol. 25, no. 4, pp. 4028–4041, 2023.
A. Agung, P. Sidhiawan, E. Hidayati, and B. F. Suryadi, “Eksplorasi Potensi Bacillus spp. sebagai Bakteri Pemacu Pertumbuhan Tanaman di Hutan Primer Resort Kembang Kuning,” J. Ilm. Biol., vol. 11, no. 2, pp. 1017–1029, 2023. DOI: https://doi.org/10.33394/bioscientist.v11i2.8403
M. W. Khalifa, N. Rouag, and M. Bouhadida, “Evaluation of the Antagonistic Effect of Pseudomonas Rhizobacteria on Fusarium Wilt of Chickpea,” Agriculture, vol. 12, no. 3, pp. 1–16, 2022, doi: 10.3390/agriculture12030429. DOI: https://doi.org/10.3390/agriculture12030429
F. Marsaoli, J. M. Matinahoru, and C. Leiwakabessy, “Isolasi, Seleksi, dan Uji Antagonis Bakteri Endofit diisolasi dari Salawaku (Falcataria mollucana) dalam Menekan Pertumbuhan Cendawan Patogen Cercospora spp,” Agrologia, vol. 8, no. 2, pp. 44–54, 2020, doi: 10.30598/a.v8i2.1009. DOI: https://doi.org/10.30598/a.v8i2.1009
C. P. Lin and Y. C. Ho, “Beneficial microbes and basal fertilization in antagonism of banana fusarium wilt,” Agronomy, vol. 11, no. 10, pp. 1–19, 2021, doi: 10.3390/agronomy11102043. DOI: https://doi.org/10.3390/agronomy11102043
G. Bubici, M. Kaushal, M. I. Prigigallo, C. G. L. Cabanás, and J. Mercado-Blanco, “Biological Control Agents Against Fusarium Wilt of Banana,” Front. Microbiol., vol. 10, no. 616, pp. 1–33, 2019, doi: 10.3389/fmicb.2019.00616. DOI: https://doi.org/10.3389/fmicb.2019.00616
PDF Downloads: 72
Published
How to Cite
Write scientific names with Italic fonts:
Issue
Section
Copyright (c) 2024 Anggit Cahyani, Harman Hamidson, Suwandi Suwandi, Abu Umayah
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
d. Authors hold the copyright and retain publishing right of articles without restrictions.
.gif)
