ENDOPHYTIC TESTING OF Serratia marcescens strain NPKC3_2_21 AGAINST INPARA 3 RICE VARIETY
DOI:
https://doi.org/10.24233/biov.9.1.2023.372Keywords:
Endophytic bacterium , cellulolytic , ligninolytic , rice endophyte , swamp rice plantAbstract
Pest management is a crucial concern, especially when dealing with insect pests that can cause extensive damage to agricultural crops and the economy. One such pest is the rice stem borer (Scirpophaga innotata), which infests rice stems and poses a significant threat. To combat these pests, various microbial agents have been developed for effective pest control. Among these, the endophytic microbe in biological plant protection plays a key role in the program of sustainable pest management. An endophytic bacterium, Serratia marcescens strain NPKC3_2_21, has been identified as a key player in sustainable pest management, particularly in rice crops. In this study, we aimed to investigate the endophytic characteristics of S. marcescens strain NPKC3_2_21 in the swamp rice plant of the Inpara 3 variety of swamp rice plants. To establish its roles. Marcescens as an endophytic bacterium in rice plants, specifically the Inpara 3 variety, we conducted tests by inoculating bacteria on the plant tissue of rice plants that have been sterilized rice plant tissues with the bacteria. We assessed the presence of S. marcescens strain NPKC3_2_21 in plant tissues by applying isolates to the surface of rock wool, which supported the growth of wet rice plants aged ten or over ten days or older after planting. Samples were collected from the underside of the stem, the bottom of the leaves, and the roots on days 2, 7, and 14 after the application of isolates to the rock wool. The samples were then washed in 70% alcohol and 4% chlorox for 30 seconds and subsequently isolated on Luria Bertani (LB) agar media. Furthermore, we conducted tests to determine the ligninolytic, cellulolytic, and proteolytic activities of S. marcescens, which helped elucidate its endophytic ability. Based on the result, the endophytic capabilities. Based on the results, we found that S. marcescens strain NPKC3_2_21 exhibited endophytic characteristics solely in the stem tissue of the Inpara 3 rice variety. However, we did not observe its presence in the root and leaf tissues.
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References
Abreo, E. and Altier, N. 2019. Pangenome of Ser-ratia marcescens strains from nosocomial and en-vironmental origins reveals different populations and the links between them. SCIENTIFIC RE-PORTS, 9(1): 1–8. ISSN: 20452322. DOI: 10.1038/s41598-018-37118-0
Afzal, I. Shinwari, Z.K. Sikandar, S. and Shahzad, S. 2019. Plant beneficial endophytic bacteria: Mechanisms, diversity, host range and genetic de-terminants.MICROBIOLOGICAL RESEARCH, 221: 36–49. ISSN: 09445013. DOI: 10.1016/j.micres.2019.02.001
Chandra, R. and Chowdhary, P. 2015. Properties of bacterial laccases and their application in bio-remediation of industrial wastes. ENVIRONMEN-TAL SCIENCES, 17(2): 326–342. ISSN: 20507895. DOI: 10.1039/c4em00627e
Chaurasia, B. 2019. Biological pretreatment of lignocellulosic biomass (Water hyacinth) with dif-ferent fungus for Enzymatic hydrolysis and Bio-ethanol production Resource: advantages, Future work and Prospects. ACTA SCIENCES AGRICUL-TURE, 5(3).
Chebotar, V.K. Malfanova, N.V. Shcherbakov, A.V. Ahtemova, G.A. Borisov, A.Y. Lugtenberg, B. and Tikhonovich, I.A. 2015. Endophytic bacte-ria in microbial preparations that improve plant development (review). APPLIED BIOCHEMISTRY AND MICROBIOLOGY, 51(3): 271–277. ISSN: 00036838. DOI: 10.1134/S0003683815030059
Cooney, S. O’Brien, S. Iversen, C. and Fanning, S. 2014. Bacteria: Other Pathogenic Enterobacteri-aceae - Enterobacter and Other Genera. ENCYLO-PEDIA OF FOOD SAFETY, Vol. 1: 433-441. ISBN: 9780123786128. DOI: 10.1016/B978-0-12-378612-8.00104-9
Devi, K.A. Pandey, P. and Sharma, G.D. 2016. Plant Growth-Promoting Endophyte Serratia mar-cescens AL2-16 Enhances the Growth of Achy-ranthes aspera L., a Medicinal Plant. HAYATI JOURNAL OF BIOSCIENCES, 23(4): 173–180.ISSN: 20864094. DOI: 10.1016/j.hjb.2016.12.006
Dewa, G.W.S and Listihani. 2021. Screening of Endophytic Bacteria Isolated from Mimosa pudica in Bali Island. SEAS (SUSTAINABLE ENVIRON-MENT AGRICULTURAL SCIENCE), 5(1): 50–57. DOI: 10.22225/seas.5.1.3303.50-57
Fekrirad, Z. Kashef, N. and Arefian, E. 2019. Photodynamic inactivation diminishes quorum sensing-mediated virulence factor production and biofilm formation of Serratia marcescens. WORLD JOURNAL OF MICROBIOLOGY AND BIOTECH-NOLOGY, 35(12): 1–9. ISSN: 15730972. DOI: 10.1007/s11274-019-2768-9
Fodor, A. Palkovics, L. and Végh, A. 2022. First report of Serratia marcescens from oleander in Hungary. PHYTOPATHOLOGIA MEDITERRA-NEA, 61(2): 311–317. ISSN: 15932095. DOI: 10.36253/phyto-13354
Gyaneshwar, P. James, E.K. Mathan, N. Red-dy, P. M. Reinhold-Hurek, B. and Ladha, J.K. 2001. Endophytic colonization of rice by a diazo-trophic strain of Serratia marcescens. JOURNAL OF BACTERIOLOGY, 183(8): 2634–2645. ISSN: 00219193.DOI: 10.1128/JB.183.8.2634-2645.2001
Haddix, P.L. and Shanks, R.M.Q. 2018. Prodi-giosin pigment of Serratia marcescens is associat-ed with increased biomass production. ARCHIEVES OF MICROBIOLOGY, 200(7): 989–999. ISSN: 1432072X. DOI: 10.1007/s00203-018-1508-0
Jupatanakul, N. Pengon, J. Selisana, S.M.G. Choksawangkarn, W. Jaito, N. Saeung, A. Bun-yong, R. Posayapisit, N. Thammatinna, K. Kal-pongnukul, N. Aupalee, K. Pisitkun, T. and Kam-chonwongpaisan, S. 2020. Serratia marcescens secretes proteases and chitinases with larvicidal activity against Anopheles dirus. ACTA TROPICA, 212(3): 105686. ISSN: 18736254. DOI: 10.1016/j.actatropica.2020.105686
Kaga, H. Mano, H. Tanaka, F. Watanabe, A. Kaneko, S. and Morisaki, H. 2009. Rice seeds as sources of endophytic bacteria. MICROBES AND ENVIRONMENTS, 24(2): 154–162.ISSN: 13426311. DOI: 10.1264/jsme2.ME09113
Kalayu, G. 2019. Phosphate solubilizing mi-croorganisms: Promising approach as biofertiliz-ers. INTERNATIONAL JOURNAL OF AGRONO-MY, 2019: -. ISSN: 16878167. DOI: 10.1155/2019/4917256
Khaeruni, A. Nirmala, T. Siti, A.H.W. Gusnawaty, G. Wijayanto, T. and Sutariati, K.G.A. 2020. Potensi dan Karakterisasi Fisiologis Bakteri Endofit Asal Tanaman Kakao Sehat sebagai Pem-acu Pertumbuhan Benih Kakao. JURNAL ILMU PERTANIAN INDONESIA. 25(3): 388–395. ISSN: 0853-4217. DOI: 10.18343/jipi.25.3.388
Kumar, A. and Chandra, R. 2020. Ligninolytic enzymes and its mechanisms for degradation of lignocellulosic waste in environment. HELIYON, 6(2): e03170. ISSN: 24058440. DOI: 10.1016/j.heliyon.2020.e03170
Kumar, D.M. Lawrence, L. Rajan, R. Pri-yadarshini, S. Yachittybabu, S. and Kalaichelvan, P.T. 2012. Characterization of lipase and protease from Serratia marcescens DEPTK21 and its des-taining capability. ASIAN JOURNAL OF EXPERI-MENTAL BIOLOGICAL SCIENCES, 3(3): 621-628.
Li, Y. Guo, L. Haggblom, M.M. Yang, R. Li, M. Sun, X. ... and Sun, W. 2022. Serratia spp. are responsible for nitrogen fixation fueled by As (III) oxidation, a novel biogeochemical process identi-fied in mine tailings. ENVIRONMENTAL SCIENCE AND TECHNOLOGY, 56(3): 2033-2043. DOI: 10.1021/acs.est.1c06857
Mahlen, S.D. 2011. Serratia infections: From military experiments to current practice. CLINI-CAL MICROBIOLOGY REVIEWS, 24(4): 755–791. DOI: 10.1128/CMR.00017-11
Martínez-Zavala, S.A. Barboza-Pérez, U.E. Hernández-Guzmán, G. Bideshi, D.K. and Barbo-za-Corona, J.E. 2020. Chitinases of Bacillus thu-ringiensis: Phylogeny, Modular Structure, and Ap-plied Potentials. FRONTIERS IN MICROBIOLO-GY, 10(1): 1–15. ISSN: 1664302X. DOI: 10.3389/fmicb.2019.03032
Mathur, P., P. Mehtani, and C. Sharma. 2021. Leaf endophytes and their bioactive compounds. In: Shrivastava, N., S. Mahajan, A. Varma (Ed), Symbiotic Soil Microorganisms.
Nair, D.N. and Padmavathy, S. 2014. Impact of endophytic microorganisms on plants, envi-ronment and humans. THE SCIENTIFIC WORLD JOURNAL, 2014. ISSN: 1537744X. DOI: 10.1155/2014/250693
Nascente, A.S. Lanna, A.C. de Sousa, T.P. Chaibub, A.A. de Souza, A.C.A. and de Filippi, M.C.C. 2019. N Fertilizer Dose-Dependent Effi-ciency of Serratia spp. for Improving Growth and Yield of Upland Rice (Oryza sativa L.). INTER-NATIONAL JOURNAL OF PLANT PRODUCTION, 13(3): 217–226. ISSN: 17358043. DOI: 10.1007/s42106-019-00049-5
Niu, H, Sun, Y. Zhang, Z. Zhao, D. Wang, N. Wang, L. and Guo, H. 2022. The endophytic bac-terial entomopathogen Serratia marcescens pro-motes plant growth and improves resistance against Nilaparvata lugens in rice. MICROBIOLOGICAL RESEARCH, 256: 126956. DOI: 10.1016/j.micres.2021.126956
Purkayastha, G.D. Mangar, P. Saha, A. and Sa-ha, D. 2018. Evaluation of the biocontrol efficacy of a Serratia marcescens strain indigenous to tea rhizosphere for the management of root rot dis-ease in tea. PLOS ONE. 13(2): 1–27. ISSN: 19326203. DOI: 10.1371/journal.pone.0191761
Raymann, K. Coon, K.L. Shaffer, Z. Salisbury, S. and Moran, N.A. 2018. Pathogenicity of serratia marcescens strains in honey bees. MBio. 9(5): 1–15. ISSN: 21507511. DOI: 10.1128/mBio.01649-18
Sutio, G. Azzahra, N.A. Maharani, R. and Bas-ri, M. 2023. Serratia marcescens strain NPKC3_2_21 as endophytic phosphate solubiliz-ing bacteria and entomopathogen: Promising com-bination approach as rice biofertilizer and bi-opesticide. BIODIVERSITAS, 24(2): 901–909. ISSN: 20854722. DOI: 10.13057/biodiv/d240228
Tang, H. Li, Y.Q. Zheng, L. Wang, M.J. and Luo, C.B. 2022. Efficient saccharification of bamboo biomass by secretome protein of the cel-lulolytic bacterium Serratia marcescens LY1 based on whole-genome and secretome analy-sis. RENEWABLE ENERGY, 193: 32-40. DOI: 10.1016/j.renene.2022.04.146
Thapa, S. Mishra, J. Arora, N. Mishra, P. Li, H. O′Hair, J. Bhatti, S. and Zhou, S. 2020. Microbial cellulolytic enzymes: diversity and biotechnology with reference to lignocellulosic biomass degrada-tion. REVIEWS IN ENVIRONMENTAL SCIENCE AND BIOTECHNOLOGY, 19(3): 621–648. ISSN: 15729826. DOI: 10.1007/s11157-020-09536-y
Ummah, R. Asri, M.T. and Yakub, P. 2019. The Potency of Endophytic Bacteria Isolates in Onion (Allium ascalonicum) Roots as Nitrogen Fixer. LENTERABIO, 8(2): 143–149. http://ejournal.unesa.ac.id/index.php/lenterabio
Varma, K. Pavuluri, K. Uppala, L.S, and Chapala, M.M. 2017. Endophytes: role and func-tions in crop health. In D.P. Singh et al. (Eds.). Plant-Microbe Interactions in Agro-Ecological Perspectives. DOI: 10.1007/978-981-10-5813-4_15
Walia, A. Guleria, S. Chauhan, A. and Mehta, P. 2017. Endophytic bacteria: role in phosphate solubilization. Endophytes. CROP PRODUCTIVI-TY AND PROTECTION, 2(16): 61-93. DOI: 10.1007/978-3-319-6
White, J.F. Kingsley, Kathryn, L.Z. Qiuwei, V. Rajan, O. Nkolika, D. Sofia, E. Matthew, T. Ver-ma, S.K. Gond, S.K. and Kowalski, K.P. 2019. Review: Endophytic microbes and their potential applications in crop management. PEST MAN-AGEMENT SCIENCE, 75(10): 2558-2565. ISSN: 15264998. DOI: 10.1002/ps.5527
Xia, Y. Liu, J. Chen, C. Mo, X. Tan, Q. He, Y. Wang, Z. Yin, J. Zhou, and G. The Multifunctions and Future Prospects of Endophytes and Their Metabolites in Plant Disease Management. MI-CROORGANISM, 10(5): 1-19. ISSN: 20762607. DOI: 10.3390/ microorganisms1005107
Zinniel, D.K. Lambrecht, P. Harris, N.B. Feng, Z. Kuczmarski, D. Higley, P. Ishimaru, C.A. Aru-nakumari, A. Barletta, R.G. and Vidaver, A.K. 2022. Isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plants. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(5):2198-2208. ISSN: 00992240
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