THE ANTIBACTERIAL ACTIVITY OF WATER APPLE LEAVES ACTIVE COMPOUND (Syzygium zeylanicum) AGAINST Escherichia coli AND Staphylococcus aureus
DOI:
https://doi.org/10.24233/BIOV.3.1.2017.54Abstract
Escherichia coli is one of the bacteria that cause infections in the human digestive tract such as diarrhea, while Staphylococcus aureus is one of the bacteria that cause infections in the skin injury such as boils and pimples. This study used Syzygium zeylanicum leaves because it has potential as a antibacterial because it contains active compounds. This study aimed was determine the antibacterial activity of the fraction and the active compound in Syzygium zeylanicum leaves against Escherichia coli and Staphylococcus aureus. Research conducted on November 2015 to January 2016. The method used in this research were extraction by maceration, fractionation by liquid fractionation, antibacterial activity test, and determination of minimum inhibitory concentration with the diffusion method and isolation of active compounds by column chromatography method. The bacteria used in this test are Escherichia coli and Staphylococcus aureus. Data are presented in tabular form based on the average value of the inhibition diameter and deviation standard. The results of this research showed the water methanol active fraction against the bacteria that used in this test. The methanol water fraction had obtained one antibacterial compound in bottle 1,3,5 which shows the value of tannin Rf 0,416. The minimum inhibitory concentration of water methanol of water apple leaves is 1000 µg/mL for Escherichia coli and 500 µg/mL for Staphylococcus aureus. The minimum inhibitory concentration of the active compound to Escherichia coli and Staphylococcus aureus in 500 µg/mL. The fraction and the active compound of water apple leaves have an antibacterial activity with Escherichia coli and Staphylococcus aureus and the active compound is tannin.
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References
Aini, N, H., Saleh, C., dan Erwin. 2015. Uji Toksisitas Dan Aktivitas Antibakteri Ekstrak Daun Merah Tanaman Pucuk Merah (Syzygium myrtifolium Walp.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli. Jurnal Kimia Mulawarman.
(13) :35-39 hlm.
Anoop, M, V., Bindu, A, R. 2014. Pharmacognostic and Physico-Chemical Studies on Leaves of Syzygium zeylanicum (L.) DC. International Journal of Pharmacognosy and Phytochemical Research. 6(4) :685-689 hlm.
Bridson, E, Y. 1998. The Oxoid Manual. Wade Road, Basingstoke, Hampshire. England:
+371 hlm.
Davis , W, W., and Stout, T, R. 1971. Disc Plate Method Of Microbiological Antibiotic
Assay. Journal Of Microbiology. 22(4) :659-665 hlm.
Darwis, W., Romauli, S., dan Kasrina. 2013. Uji Efektivitas Ekstrak Daun Iler-Iler (Coleusscutellarioides (Linn)(Benth) Sebagai Antibakteri Staphylococcus aureus. Konservasi Hayati. 9(2) :55-59 hlm.
Ganiswarna, S, G. 1995. Farmakologi dan Terapi Edisi 4. Jakarta: UI-Fakultas Kedokteran
:208-211 hlm.
Harbone, J, B. 1987. Metode Fitokimia. Diterjemahkan oleh Kosasih Padmawinata dan
Iwang Sudiro. Bandung :Penerbit ITB.
Holetz, F, B., Pessini, G, L., Sanchez, D., Aparicio, G. Cortez, C, V. Nakamura., dan Filho, B, P, D. 2002. Screening of Some Plants Used in The Brazillian Folk Medicine For The Treatment of Infections I. Journal of Bioline International .http://www.bopline- org.br/request02229.
Jawetz, E., Mellnick, J, L., Adellberg, E, A. 2005. Mikrobiologi Kedoteran. Edisi 20.
Jakarta. EGC :37-40 hlm.
Kusmayati dan Agustini, N. W. R. 2007. Uji Aktivitas Senyawa Antibakteri dari
Mikroalga (Porphyridium cruentum). Biodiversitas. 8(1) :48-53 hlm.
Mulyani, Y., Bachtiar, E., dan Untung, M, K, A., 2013. Peranan Senyawa Metabolit Sekunder Tumbuhan Mangrove Terhadap Infeksi Bakteri Aeromonas hydrophila pada Ikan Mas (Cyprinus carpio L.). Jurnal Akuatika. 4(1) :1-9 hlm.
Nurdin, A M., Titin, S, F, M., dan Zackiyah. 2010. Penentuan Pelarut Terbaik Dalam Mengekstraksi Senyawa Bioaktif Dari Kulit Batang Artocarpus heterophyllus. Jurnal Sains dan Teknologi Kimia. 2(1) :150-158 hlm.
Rahmawati, F., Dwiyanti, G., dan Sholihin, H. 2013. Kajian Aktivitas Antioksidan Produk Olahan Buah Jambu Biji Merah (Psidium guajava L.). Jurnal Sains dan Teknologi. 1(4) :1-8 hlm.
Rosaidah dan Mahita, A, W. 2012.Potensi Ekstrak Daun Jambu Biji Sebagai Antibakterial Untuk Menanggulangi Serangan Bakteri Aeromonas hydrophila Pada Ikan Gurame (Osphronemus gourami lacepede). Jurnal Akuatika. 1(3) :19-27 hlm.
Salni, Aminasih, N., Sriviona, R. 2013. Isolasi Senyawa Antijamur dari Rimpang Lengkuas Putih (Alpinia galangal (L.) Willd) dan Penentuan Konsentrasi Hambat Minimum Terhadap Candida albicans. Prosiding Semirata FMIPA Universitas Lampung.
Salni, Marisa, H., Wedya, M, R. 2011. Isolasi Senyawa Antibakteri Dari Daun Jengkol (Pithecolobium lobatum Benth) dan Penentuan Nilai KHM. Jurnal Penelitian Sains. 14(1) :1-4 hlm.
Tinambunan, H., Melki., dan Isnaini. 2012. Efektivitas Ekstrak Bakteri yang Berasosiasi Dengan Spons dan Karang Lunak Sebagai Antibakteri dari Perairan Pulai Tegal Lampung. Maspari Journal. 4(2) :225-230 hlm.
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