CONDITION OPTIMIZATION PRODUCTION XYLANASE THERMOSTABLE BY Bacillus licheniformis TS10 USING SUBSTRATE OIL PALM EMPTY FRUIT BUNCHES
DOI:
https://doi.org/10.24233/BIOV.3.1.2017.51Abstract
Optimization conditions for thermostable xylanase production of Bacillus licheniformis TS10 by using substrate oil palm empty fruit bunches has been conducted from November 2015 to January 2016 in the Laboratory of Genetics and Biotechnology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya. The aims of this study was to determine the potential of oil palm empty fruit bunches ( EFB ) as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10 and the optimum conditions of temperature, pH and substrate concentration in the fermentation process. The research method is to make the growth curve and the curve of Bacillus licheniformis TS10 enzyme production on EFB substrate. The number of bacterial cells was determined by using a UV-VIS spectrophotometer and total plate count (TPC) through a standard curve. Optimization performed at various pH (5, 6, 7, 8, 9), temperature (50 °C, 60 °C, 70 °C, 80 °C) and substrate (1%, 2%, 3%, 4%) , Test activity at each pH, temperature and substrate using methods DNS by measuring enzyme activity based on the reducing sugar released by the substrate by using dinitrosalicylic acid (DNS). Based on the results of this research showed that oil palm empty fruit bunches (EFB ) has potential as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10, thermostable xylanase production of Bacillus licheniformis TS10 on the substrate EFB has an optimum condition at pH 6, 80 °C and the substrate concentration of 4%.Last Year PDF Downloads
References
Akhdiya, A. 2003. Isolasi Bakteri Penghasil Enzim Protease Alkalin Termostabil. Buletin Plasma Nutfah. 9(2). Balai Penelitian Bioteknologi dan Sumber Daya Genetik Pertanian. Bogor.
Bailey, M. J. 1992. Interlaboratory testing of methods for assay of xylanase activity. Jurnal Biotechnology. 23: 257-270.
Beg, Q. K., Kapoor, M., Mahajan, L., Hoodal, G. S. 2001. Microbial Xylanases and their Industrial application: A Review. Apply Microbiology Biotechnology. 56: 326-338.
Hastari, A. A., Mahdi, C., Sutrisno. 2014. Penentuan Waktu Fermentasi Optimum Produksi Xilanase Dari Trichoderma Viride Menggunakan Substrat Kulit Pisang Dan Kulit Melon Dengan Fermentasi Semi Padat. Kimia Student Journal. 1(1): 119-125.
Ilmi, I. M., Kuswytasari, N. D. 2013. Aktifitas Enzim Lignin Peroksidase oleh Gliomastix sp. T3.7 pada Limbah Bonggol Jagung dengan Berbagai pH dan Suhu. Jurnal Sains Dan Seni Pomits. 2(1): 1-5.
Kulkarni, N. Shendy, A. And Rao, M. 1999. Molecular and Biotechnological aspects of xylanases. FEMS Microbiology Review. 23 : 411-456.
Li, M.F., Fan, Y.M., Sun, R.C. & Xu,F. 2010. Characterization of extracted lignin of bamboo (Neosinocalamus affinis) preteaded with sodium hydroxide/urea solution at low temperature. Bioresources 5(3): 1762-1778.
Muharni, Yohandini, H. dan Hermansyah. 2013. Eksplorasi Enzim Xilanase Termostabil : Kloning Gen Penyandi Xilanase Dari Bakteri Termofilik. Laporan Penelitian. Universitas Sriwijaya.
Murni, S. W., Kholisoh, S. D., Tanti, D. L., Petrissia, E. M. 2011. 2011. Produksi, Karakterisasi, dan Isolasi Lipase dari Aspergillus niger. Prosiding Seminar Nasional Teknik Kimia “Kejuangan”. Yogyakarta.
Nareswari, A., 2007. Enzim Xilanase Bacillus licheniformis AQ1: Pemekatan, Studi Thermostabilitas, dan Zimogram. Skripsi. Institut Pertanian Bogor. 1-29.
Puspaningsih, N. N. T., Suwito, H., Sumarsih, S., Rohman, A., Aemarani, O. 2007. Hidrolisis Beberapa Jenis Xilan Dengan Enzim Xilanolitik Termofilik Rekombinan. Berkas Penelitian Hayati.12(1): 11-5.
Putra, A. I., Wahyuntari, B., Aulanni’am. 2012. Optimasi Produksi Xilanase Oleh B. subtilis AQ1 Menggunakan Response Surface Methodology. Jurnal Saainstek. 4(2): 1-7.
Safaria, S., Nora, I., Zaharah, T. A. 2013. Efektivitas Campuran Enzim Selulase Dari Aspergillus niger dan Trichoderma reesei Dalam Menghidrolisis Substrat Sabut Kelapa . JKK. 2(1): 1-6.
Saragih, B. A., 2013. Skrining Bakteri Pelarut Fosfat Adaptif Vinasse Dari Lahan Tebu Pabrik Gula Jatiroto Kabupaten Lumajang Jawa Timur. Skripsi. Universitas Jember. 1-56.
Septiningrum, K., Chandra, A. P. 2011. Produksi Xilanase Dari Tongkol Jagung Dengan Sistem Bioproses Menggunakan Bacillus Circulans Untuk Pra-Pemutihan Pulp. Jurnal Riset Industri. Kementerian Perindustrian Indonesia. 5(1): 1-11.
Septiningrum, K., Moeis, M. R. 2009. Isolasi Dan Karakterisasi Xilanase dari Bacillus circulans. Bs. 44(1): 31-40.
Soeka, Y. S., Rahayu, S. H., Setianingrum, N., Naiola, E. 2011. Kemampuan Bacillus Licheniformis dalam Memproduksi Enzim Protease Yang Bersifat Alkalin Dan Termofilik. Artikel. Media Litbang Kesehatan. 21(2): 1-7.
Sriyapai, T., Somyoonsap, P., Matsui, K., Kawai, P., Chansiri, K. 2011. Cloning of a thermostablr xylanase from Actinomadura sp. S14 and its expression in Escherichia coli and Phicia pastoris Jurnal biosciense. 111(5): 528-536.
Supriyanto, A. 2009. Manfaat Jamur Pelapuk Putih Phanerochaete Chrysosporium L1 Dan Pleurotus EB9 Untuk Biobleaching Pulp Kardus Bekas. Skripsi. Institut Pertanian Bogor.
Susilowati, P. E., Raharjo, S., Kurniawati, D., Rahim, R., Sumarlin, Ardiansyah. 2012. Produksi Xilanase dari Isolat Sumber Air Panas Sonai, Sulawesi Tenggara, menggunakan Limbah Pertanian. Jurnal Natur Indonesia. 14(3): 1-6.
Trismilah, Waltam, D. R. 2009. Produksi Xilanase Menggunakan Media Limbah Pertanian Dan Perkebunan. Jurnal Teknik Lingkungan.10(2): 137 – 144.
Wibowo, S. G. 2014. Optimasi Produksi Xilanase dari Bacillus halodurans CM1 Dengan Metode Permukaan Respon. Tesis. Institut Pertanian Bogor. 1-59.
Yuniar, W. 2013. Skrining Dan Identifikasi Kapang Selulolitik Pada Proses Vermikomposting Tandan Kosong Kelapa Sawit (TKKS). Skripsi. Universitas Jember. 1-53.
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