A SIMPLE PROTOCOL FOR SOMATIC EMBRYOGENESIS INDUCTION OF IN VITRO SUGARCANE ( Saccharum officinarum. L) BY 2,4-D AND BAP

Authors

  • Laily Ilman Widuri sriwijaya university
  • Parawita Dewanti Faculty of Agriculture, University of Jember. Center for Development of Advanced Science and Technology (CDAST), University of Jember
  • Bambang Sugiharto Faculty of Mathematics and Natural Science, University of Jember. Center for Development of Advanced Science and Technology (CDAST), University of Jember

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DOI:

https://doi.org/10.24233/BIOV.2.1.2016.32

Abstract

Induction of in vitro sugarcane through somatic embryogenesis technique influenced by addition of plant growth regulator. The objective of this research was to determine appropriate formulation medium for indirect somatic embryogenesis induction on two potential sugarcane SUT Event 02 and PS 881. This research carried out in three steps, callus induction, callus proliferation, and shoot regeneration. Explants taken from basal of in vitro plantlet one month SUT Event 02 and PS 881 resulted from shoot regeneration previously. Five different medium formulas applied for callus induction and one formula for proliferation and shoot regeneration. Research using completely randomized design (CRD) factorial with five different formulation induction mediums. The result showed that the best respond of indirect somatic embryogenesis on SUT Event 02 and PS 881 was medium containing  3 mgL-1of  2,4-D.

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References

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Published

31-05-2016

How to Cite
Write scientific names with Italic fonts:

Widuri, L. I., Dewanti, P., & Sugiharto, B. (2016). A SIMPLE PROTOCOL FOR SOMATIC EMBRYOGENESIS INDUCTION OF IN VITRO SUGARCANE ( Saccharum officinarum. L) BY 2,4-D AND BAP. BIOVALENTIA: Biological Research Journal, 2(1), 1–9. https://doi.org/10.24233/BIOV.2.1.2016.32

Issue

Vol. 2 No. 1 (2016)

Section

Vol 2, No 1 (2016): May 2016

Copyright (c) 2016 Laily Ilman Widuri, Parawita Dewanti, Bambang Sugiharto

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