A In Vitro Callus Induction from (Antidesma bunius L.) Leaves
DOI:
https://doi.org/10.24233/biov.11.1.2025.451Keywords:
Buni leaves , In Vitro , Callus , ZPTAbstract
Antidesma bunius L. is a tree species that has ecological and economic benefits, and has many bioactivities. Propagation of this tree can be done in several ways, one of which is callus induction. Callus is a collection of undifferentiated cells that can develop into new plants with the help of ZPT. The purpose of this study was to determine the effect and optimum combination of ZPT 2,4-D and BAP on the induction of leaf callus of A. bunius L. The research design was a non-factorial Completely Randomised Design (CRD) with 4 treatments namely P0: MS without the addition of zpt; P1: MS + 1 ppm BAP + 3 ppm 2,4-D; P2: MS + 2 ppm BAP + 2 ppm 2,4-D; P3: MS + 3 ppm BAP + 1 ppm 2,4-D. Parameters observed included callus emergence time, percentage of callus explants, and callus morphology. The results showed that P2 is the best concentration for callus induction, by looking at the observed effect on callus emergence time of 6 HST, percentage of callus explants 56% and callus morphology which is white with crumb texture, which can develop into embryogenic callus
Last Year PDF Downloads
References
Lim, T. K. (2012). Antidesma bunius, in Edible Medicinal and Non-Medicinal Plants Volume 4 Fruits. Springer, pp. 220-224. doi: 10.1007/978-94-007-4053-2_27.
Lawag, I. L., Aguinaldo, A. M., Naheed, S., and Mosihuzzaman, M. (2012). α-Glucosidase Inhibitory Activity of Selected Philippine Plants. J. Ethnopharmacol. 144(1), pp. 217–219.doi:10.1016/j.foodchem.2015.02.093.
Krongyut, O., and Sutthanut, K. (2019). Phenolic Profile, Antioxidant Activity, and Anti-obesogenic Bioactivity of Mao Luang Fruits (Antidesma bunius L.). Molecules, 24(22), pp.4109. doi: 10.3390/molecules24224109.
Sulfiani, E., Aswadi, D. M. R., Tapelo, S.R., Yusuf, L., and Slamet, S. (2022). Sirup Bucin : Minuman Herbal Kekinian Kombinasi Sari Buah Buni , Sereh dan Jahe Sebagai Alternatif Minuman Kesehatan Kaya Antioksidan. Jambura Journal of Health Sciences and Research. pp.1-6 Available:https://ejurnal.ung.ac.id/index.php/jjhsr/index%0D
Ong, H. G., and Kim, Y.D. (2017). The Role of Wild Edible Plants in Household Food Security Among Transitioning Hunter-Gatherers: Evidence from the Philippines. Food Secur, 9(1), pp. 11–24. doi: 10.1007/s12571-016-0630-6.
Sari, N., Rahayu, E.S., and Sumadi. (2014). Optimasi Jenis dan Konsentrasi ZPT dalam Induksi Kalus Embriogenik dan Regenerasi menjadi Planlet pada Carica pubescens (Lenne & K.Koch). Biosaintifika Journal of Biology & Biology Education. 6(1), pp. 51–59. [Online]. Available: http://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/3785
Prihastanti, E., Hastuti, E.D., and Suedy, S.W.A. (2020). Short Communication: Comparing the Growth of Stem Explants between Citrus reticulata var. Tawangmangu and C. reticulata var. garut Using In vitro Culture Methods. Biodiversitas,21(12), pp.5845–5849. doi: 10.13057/biodiv/d211248.
Tarigan, S.D.S., Astarini, I. A., and Astiti, N.P.A. (2023). Inisiasi Kalus Bangle (Zingiber purpureum Roscoe) pada Beberapa Kombinasi 2.4-D dan Kinetin. J. Hortik. Indones, 14(2), pp. 93–99. doi: 10.29244/jhi.14.2.93-99.
Waryastuti, D.E., Setyobudi, L., and Wardiyati, T. (2017). Pengaruh Tingkat Konsentrasi 2,4-D dan BAP pada Media MS terhadap Induksi Kalus Embriogenik Temulawak (Curcuma xanthorrhiza Roxb .). J. Produksi Tanam, 5(1), pp.140–149.
Kona, P., Kumar, M.H., Reddy, K.H.P., Reddy, D.M., Eswar Reddy, N.P., Latha, P., and Balaji, M.S. (2019). Effect of 2,4- D and EMS On in vitro Regeneration in Sugarcane Cultivar, Co86032. International Journal Current Microbiology and Applied Sciences, 8(3), pp.1228–1236. doi: 10.20546/ijcmas.2019.803.145.
Dar, S.A., Nawchoo, I.A., Tyub, S., and Kamili, A.N. (2021). Effect of Plant Growth Regulators on In Vitro Induction and Maintenance of Callus from Leaf and Root Explants of Atropa acuminata Royle ex Lindl. Biotechnol. Reports, 32, p.e00688. doi: 10.1016/j.btre.2021.e00688.
George, E.F., Hall, M.A., and De Klerk, G-J. (2008). Plant Propagation by Tissue Culture 3rd Edition. Netherlands. http://dx.doi.org/10.1007/978-1-4020-5005-3_8
Schaller, G.E., Bishopp, A., and Kieber, J.J. (2015). The Yin-Yang of Hormones: Cytokinin and Auxin Interactions in Plant Development. Plant Cell, 27(1), pp. 44–63. doi: 10.1105/tpc.114.133595.
Nurokhman, A., Faizah, H., Sugiharto, Utami, E.S.W., and Manuhara, Y.S.W. (2019). Effect of Plant Growth Regulator and Explant Types on In Vitro Callus Induction of Gynura procumbens (Lour.) Merr. Res. J. Biotechnol, 14(9), pp. 102–107.
Prashariska, K., Pitoyo, A., and Solichatun, S. (2021). Pengaruh Indole-3-Acetic Acid (IAA) dan Benzyl Amino Purine (BAP) Terhadap Induksi dan Deteksi Alkaloid Kalus Kamilen (Matricaria chamomilla L.). Innofarm Jurnal Inov. Pertan, 23(2), pp.104–114. doi: 10.33061/innofarm.v23i2.5916.
Ramulifho, E., Goche, T., Van As, J., Tsilo, T.J., Chivasa, S., and Ngara, R. (2019). Establishment and Characterization of Callus and Cell Suspension Cultures of Selected Sorghum bicolor (L.) Moench Varieties: A resource for gene discovery in plant stress biology. Agronomy, 9(5). doi: 10.3390/agronomy9050218.
Isda, M.N., and Salsabilla, M.J. (2022). In Vitro Callus Induction in Tacca (Tacca chantrieri Andre) Leaf Explants on Murashige and Skoog Media with Different Concentrations of Sucrose. Jurnal Biologi Universitas Andalas, 10(1), pp.1-9. DOI: https://doi.org/10.25077/jbioua.10.1.1-9.2022
Yelnititis,Y. (2012). Pembentukan kalus remah dari eksplan daun Ramin (Gonystylus bancanus (Miq) Kurz.). J. Pemuliaan Tanam. Hutan, 6(3), pp.181-194. doi: 10.20886/jpth.2012.6.3.181-194
PDF Downloads: 18
Published
How to Cite
Write scientific names with Italic fonts:
Issue
Section
Copyright (c) 2025 Fitria Y.Hs. Adju, Jusna Ahmad, Devi Bunga Pagalla, Novri Youla Kandowangko, Indriati Husain, Lindawaty Isima
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
d. Authors hold the copyright and retain publishing right of articles without restrictions.
.gif)
