A In Vitro Callus Induction from (Antidesma bunius L.) Leaves

Authors

  • Fitria Y.Hs. Adju Department of Biology, Faculty of Mathematics & Natural Sciences, Universitas Negeri Gorontalo. Jalan Prof. B. J. Habibie, Bone Bolango 96583, Gorontalo 96562, Indonesia
  • Jusna Ahmad Department of Biology, Faculty of Mathematics & Natural Sciences, Universitas Negeri Gorontalo. Jalan Prof. B. J. Habibie, Bone Bolango 96583, Gorontalo 96562, Indonesia
  • Devi Bunga Pagalla Department of Biology, Faculty of Mathematics & Natural Sciences, Universitas Negeri Gorontalo. Jalan Prof. B. J. Habibie, Bone Bolango 96583, Gorontalo 96562, Indonesia
  • Novri Youla Kandowangko Department of Biology, Faculty of Mathematics & Natural Sciences, Universitas Negeri Gorontalo. Jalan Prof. B. J. Habibie, Bone Bolango 96583, Gorontalo 96562, Indonesia
  • Indriati Husain Department of Agrotechnology, Faculty of Agriculture, Universitas Negeri Gorontalo. Jalan Prof. B. J. Habibie, Bone Bolango 96583, Gorontalo 96562, Indonesia
  • Lindawaty Isima UPTD of the Gorontalo Provincial Agricultural Seed, Supervision and Certification Center. Prof. Dr.Aloei Saboe. Kabila, Bone Bolango 96128, Gorontalo 96562, Indonesia

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DOI:

https://doi.org/10.24233/biov.11.1.2025.451

Keywords:

Buni leaves , In Vitro , Callus , ZPT

Abstract

Antidesma bunius L. is a tree species that has ecological and economic benefits, and has many bioactivities. Propagation of this tree can be done in several ways, one of which is callus induction. Callus is a collection of undifferentiated cells that can develop into new plants with the help of ZPT. The purpose of this study was to determine the effect and optimum combination of ZPT 2,4-D and BAP on the induction of leaf callus of A. bunius L. The research design was a non-factorial Completely Randomised Design (CRD) with 4 treatments namely P0: MS without the addition of zpt; P1: MS + 1 ppm BAP + 3 ppm 2,4-D; P2: MS + 2 ppm BAP + 2 ppm 2,4-D; P3: MS + 3 ppm BAP + 1 ppm 2,4-D. Parameters observed included callus emergence time, percentage of callus explants, and callus morphology. The results showed that P2 is the best concentration for callus induction, by looking at the observed effect on callus emergence time of 6 HST, percentage of callus explants 56% and callus morphology which is white with crumb texture, which can develop into embryogenic callus

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References

Lim, T. K. (2012). Antidesma bunius, in Edible Medicinal and Non-Medicinal Plants Volume 4 Fruits. Springer, pp. 220-224. doi: 10.1007/978-94-007-4053-2_27.

Lawag, I. L., Aguinaldo, A. M., Naheed, S., and Mosihuzzaman, M. (2012). α-Glucosidase Inhibitory Activity of Selected Philippine Plants. J. Ethnopharmacol. 144(1), pp. 217–219.doi:10.1016/j.foodchem.2015.02.093.

Krongyut, O., and Sutthanut, K. (2019). Phenolic Profile, Antioxidant Activity, and Anti-obesogenic Bioactivity of Mao Luang Fruits (Antidesma bunius L.). Molecules, 24(22), pp.4109. doi: 10.3390/molecules24224109.

Sulfiani, E., Aswadi, D. M. R., Tapelo, S.R., Yusuf, L., and Slamet, S. (2022). Sirup Bucin : Minuman Herbal Kekinian Kombinasi Sari Buah Buni , Sereh dan Jahe Sebagai Alternatif Minuman Kesehatan Kaya Antioksidan. Jambura Journal of Health Sciences and Research. pp.1-6 Available:https://ejurnal.ung.ac.id/index.php/jjhsr/index%0D

Ong, H. G., and Kim, Y.D. (2017). The Role of Wild Edible Plants in Household Food Security Among Transitioning Hunter-Gatherers: Evidence from the Philippines. Food Secur, 9(1), pp. 11–24. doi: 10.1007/s12571-016-0630-6.

Sari, N., Rahayu, E.S., and Sumadi. (2014). Optimasi Jenis dan Konsentrasi ZPT dalam Induksi Kalus Embriogenik dan Regenerasi menjadi Planlet pada Carica pubescens (Lenne & K.Koch). Biosaintifika Journal of Biology & Biology Education. 6(1), pp. 51–59. [Online]. Available: http://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/3785

Prihastanti, E., Hastuti, E.D., and Suedy, S.W.A. (2020). Short Communication: Comparing the Growth of Stem Explants between Citrus reticulata var. Tawangmangu and C. reticulata var. garut Using In vitro Culture Methods. Biodiversitas,21(12), pp.5845–5849. doi: 10.13057/biodiv/d211248.

Tarigan, S.D.S., Astarini, I. A., and Astiti, N.P.A. (2023). Inisiasi Kalus Bangle (Zingiber purpureum Roscoe) pada Beberapa Kombinasi 2.4-D dan Kinetin. J. Hortik. Indones, 14(2), pp. 93–99. doi: 10.29244/jhi.14.2.93-99.

Waryastuti, D.E., Setyobudi, L., and Wardiyati, T. (2017). Pengaruh Tingkat Konsentrasi 2,4-D dan BAP pada Media MS terhadap Induksi Kalus Embriogenik Temulawak (Curcuma xanthorrhiza Roxb .). J. Produksi Tanam, 5(1), pp.140–149.

Kona, P., Kumar, M.H., Reddy, K.H.P., Reddy, D.M., Eswar Reddy, N.P., Latha, P., and Balaji, M.S. (2019). Effect of 2,4- D and EMS On in vitro Regeneration in Sugarcane Cultivar, Co86032. International Journal Current Microbiology and Applied Sciences, 8(3), pp.1228–1236. doi: 10.20546/ijcmas.2019.803.145.

Dar, S.A., Nawchoo, I.A., Tyub, S., and Kamili, A.N. (2021). Effect of Plant Growth Regulators on In Vitro Induction and Maintenance of Callus from Leaf and Root Explants of Atropa acuminata Royle ex Lindl. Biotechnol. Reports, 32, p.e00688. doi: 10.1016/j.btre.2021.e00688.

George, E.F., Hall, M.A., and De Klerk, G-J. (2008). Plant Propagation by Tissue Culture 3rd Edition. Netherlands. http://dx.doi.org/10.1007/978-1-4020-5005-3_8

Schaller, G.E., Bishopp, A., and Kieber, J.J. (2015). The Yin-Yang of Hormones: Cytokinin and Auxin Interactions in Plant Development. Plant Cell, 27(1), pp. 44–63. doi: 10.1105/tpc.114.133595.

Nurokhman, A., Faizah, H., Sugiharto, Utami, E.S.W., and Manuhara, Y.S.W. (2019). Effect of Plant Growth Regulator and Explant Types on In Vitro Callus Induction of Gynura procumbens (Lour.) Merr. Res. J. Biotechnol, 14(9), pp. 102–107.

Prashariska, K., Pitoyo, A., and Solichatun, S. (2021). Pengaruh Indole-3-Acetic Acid (IAA) dan Benzyl Amino Purine (BAP) Terhadap Induksi dan Deteksi Alkaloid Kalus Kamilen (Matricaria chamomilla L.). Innofarm Jurnal Inov. Pertan, 23(2), pp.104–114. doi: 10.33061/innofarm.v23i2.5916.

Ramulifho, E., Goche, T., Van As, J., Tsilo, T.J., Chivasa, S., and Ngara, R. (2019). Establishment and Characterization of Callus and Cell Suspension Cultures of Selected Sorghum bicolor (L.) Moench Varieties: A resource for gene discovery in plant stress biology. Agronomy, 9(5). doi: 10.3390/agronomy9050218.

Isda, M.N., and Salsabilla, M.J. (2022). In Vitro Callus Induction in Tacca (Tacca chantrieri Andre) Leaf Explants on Murashige and Skoog Media with Different Concentrations of Sucrose. Jurnal Biologi Universitas Andalas, 10(1), pp.1-9. DOI: https://doi.org/10.25077/jbioua.10.1.1-9.2022

Yelnititis,Y. (2012). Pembentukan kalus remah dari eksplan daun Ramin (Gonystylus bancanus (Miq) Kurz.). J. Pemuliaan Tanam. Hutan, 6(3), pp.181-194. doi: 10.20886/jpth.2012.6.3.181-194

Published

15-04-2025

How to Cite
Write scientific names with Italic fonts:

Adju, F. Y., Ahmad, J., pagalla, D. B., Kandowangko, N. Y., Husain, I., & Isima , L. (2025). A In Vitro Callus Induction from (Antidesma bunius L.) Leaves. BIOVALENTIA: Biological Research Journal, 11(1), 40–46. https://doi.org/10.24233/biov.11.1.2025.451

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Vol 11, No 1 (2025): May 2025