DETERMINATION OF ALLANTOIN LEVELS AS A RESULT OF OPTIMIZATION OF IONIC LIQUID-ULTRASOUND ASSISTED EXTRACTION OF COMFREY LEAVES BY TLC-DENSITOMETRY
DOI:
https://doi.org/10.24233/biov.8.2.2022.306Keywords:
Allantoin , Comfrey L , IL-UAE , [HMIM]Cl , respon surface methodology , TLC-densitometryAbstract
Allantoin is a compound contained in comfrey leaves. This research aims to obtain optimal condition parameters of ionic liquid-ultrasound-assisted extraction (IL-UAE) comfrey leaves to attract allantoin compounds. Comfrey leaves are extracted with eight ionic liquids by ultrasound-assisted extraction (UAE) and screened for allantoin levels. The best ionic liquid from the screening results is optimized response surface methodology (RSM) with Box-Behnken design with three factors and three levels to determine the optimum extraction conditions for allantoin levels. Determination of levels is carried out with TLC densitometry. The results of the IL screening obtained [HMIM]Cl as the best IL in the evaluation of optimization of extraction parameters. The results of optimization of extraction condition parameters [HMIM]Cl obtained the highest allantoin levels on the sixth run of 306.396 μg / g of powder at the ratio of solvents to powders of 10 mL / g, concentrations [HMIM] Cl 1 mol / L and extraction time of 30 minutes.
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References
N. Nastić, I. Borrás-Linares, J. Lozano-Sánchez, J. Švarc-Gajić, and A. Segura-Carretero, “Compara-tive Assessment of Phytochemical Profiles of Comfrey (Symphytum officinale L.) Root Extracts Obtained by Different Extraction Techniques,” Molecules, vol. 25, no. 4, 2020, doi: 10.3390/molecules25040837.
R. Frost, S. O’Meara, and H. MacPherson, “The external use of comfrey: A practitioner survey,” Complement. Ther. Clin. Pract., vol. 20, no. 4, pp. 347–355, 2014, doi: 10.1016/j.ctcp.2014.07.003.
L. Chin, D. W. M. Leung, and H. Harry Taylor, “Lead chelation to immobilised Symphytum offic-inale L. (comfrey) root tannins,” Chemosphere, vol. 76, no. 5, pp. 711–715, 2009, doi: 10.1016/j.chemosphere.2009.04.059.
D. Janeš, B. Kalamar, and S. Kreft, “Improved method for isolation of lycopsamine from roots of comfrey (Symphytum officinale),” Nat. Prod. Commun., vol. 7, no. 7, pp. 861–862, 2012, doi: 10.1177/1934578x1200700713.
A. Trifan et al., “Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale L.) roots,” Food Chem. Toxicol., vol. 112, no. November 2017, pp. 178–187, 2018, doi: 10.1016/j.fct.2017.12.051.
N. H. Oberlies et al., “Analysis of herbal teas made from the leaves of comfrey (Symphytum officinale): reduction of N-oxides results in order of magnitude increases in the measurable concen-tration of pyrrolizidine alkaloids,” Public Health Nutr., vol. 7, no. 7, pp. 919–924, 2004, doi: 10.1079/phn2004624.
I. Indarto and A. Kirwanto, “Explorasi Metode Pengobatan Tradisional Oleh Para Pengobat Tradi-sional Di Wilayah Karesidenan Surakarta,” Inter-es. J. Ilmu Kesehat., vol. 7, no. 1, pp. 75–86, 2018, doi: 10.37341/interest.v7i1.76.
L. Kristiana, Z. K. Nantabah, and H. Maryani, “Analysis Priority of Medical Plants Potential for Emotional Mental Disorder Using Weighted Prod-uct (WP) Method: Research of Medical Plants And Herbs 2012, 2015, and 2017,” Media Penelit. Dan Pengemb. Kesehat., vol. 29, no. 3, pp. 255–268, 2019.
A. Vanitha, R. Kavinprashantha, S. Mugendhira-na, and J. Shashikanth, “Conservation Of Sym-phytum Officinale L. At Cmprh Garden, Emer-ald,” J. Univ. Shanghai Sci. Technol., vol. 24, no. 1, pp. 261–272, 2022, doi: 10.51201/jusst/22/0133.
Salehi et al., “Symphytum Species: A Comprehen-sive Review on Chemical Composition, Food Ap-plications and Phytopharmacology,” Molecules, vol. 24, no. 12, p. 2272, Jun. 2019, doi: 10.3390/molecules24122272.
C. Staiger, “Comfrey root: From tradition to mod-ern clinical trials,” Wiener Medizinische Woch-enschrift, vol. 163, no. 3–4, pp. 58–64, 2013, doi: 10.1007/s10354-012-0162-4.
J. P. Fan et al., “Optimization of ionic liquid based ultrasonic assisted extraction of puerarin from Ra-dix Puerariae Lobatae by response surface meth-odology,” Food Chem., vol. 135, no. 4, pp. 2299–2306, 2012, doi: 10.1016/j.foodchem.2012.07.038.
I. Ahmad and A. Mun’im, “Penerapan Ionic Liq-uid (Ils) sebagai Pelarut Alternatif untuk Mengekstraksi Komponen Aktif dari Tumbuhan Obat: Review,” no. April, pp. 35–52, 2016, doi: 10.25026/mpc.v3i1.65.
B. Tang, W. Bi, M. Tian, and K. H. Row, “Appli-cation of ionic liquid for extraction and separation of bioactive compounds from plants,” J. Chroma-togr. B Anal. Technol. Biomed. Life Sci., vol. 904, pp. 1–21, 2012, doi: 10.1016/j.jchromb.2012.07.020.
G. Sittampalam et al., “Assay Guidance Manual,” Assay Guid. Man., no. Md, pp. 305–336, 2016.
Hayun, “Penetapan Kadar Triprolidina Hidroklor-ida Dalam Sediaan Sirup Obat Influenza Secara Kromatografi Lapis Tipis Densitometri,” Dep. Farm. FMIPA-UI, Kampus UI Depok, vol. IV, no. 2, pp. 59–72, 2007.
K. Kimel, M. Zienkiewicz, B. Sparzak-Stefanowska, and M. Krauze-Baranowska, “TLC-densitometric analysis of allantoin in Symphytum officinale L. roots,” Acta Pharm., vol. 70, no. 1, pp. 101–110, 2019, doi: 10.2478/acph-2020-0014.
G. Zu et al., “Ultrasound-assisted extraction of carnosic acid and rosmarinic acid using ionic liq-uid solution from Rosmarinus officinalis,” Int. J. Mol. Sci., vol. 13, no. 9, pp. 11027–11043, 2012, doi: 10.3390/ijms130911027.
J. Flieger, E. B. Grushka, C.-Z. Czajkowska-, and A. Żelazko, “Ionic Liquids as Solvents in Separa-tion Processes,” Austin J Anal Pharm Chem. Aus-tin J Anal Pharm Chem, vol. 1, no. 1, pp. 1009–2, 2014.
T. Alishlah, A. Mun’Im, and M. Jufri, “Optimiza-tion of imidazolium-based ionic liquid-microwave assisted extraction for oxyresveratrol extraction from morus alba roots,” J. Young Pharm., vol. 10, no. 3, pp. 272–275, 2018, doi: 10.5530/jyp.2018.10.61.
N. Hasan, A. Mun’im, and H. Hayun, “Applica-tion of Ionic Liquids based Microwave-Assisted Extraction to Bioactive Compounds Comfrey (Symphytum officinale L.) Leaves,” J. Aisyah J. Ilmu Kesehat., vol. 7, no. 1, pp. 39–46, Mar. 2022, doi: 10.30604/jika.v7i1.764.
W. Ma, Y. Lu, R. Hu, J. Chen, Z. Zhang, and Y. Pan, “Application of ionic liquids based micro-wave-assisted extraction of three alkaloids N-nornuciferine, O-nornuciferine, and nuciferine from lotus leaf,” Talanta, vol. 80, no. 3, pp. 1292–1297, 2010, doi: 10.1016/j.talanta.2009.09.027.
X. Zhang, X. Li, Q. He, B. Zhang, and X. Zheng, “Ionic Liquid Based Ultrasonic-Assisted Extrac-tion of Oleanolic Acid from Grape Seeds,” OALib, vol. 04, no. 12, pp. 1–16, 2017, doi: 10.4236/oalib.1104148.
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