ANTIBACTERIAL ACTIVITY STUDY OF ACTIVE FRACTION FROM CHICK WEED PLANTS (Ageratum Conyzoides L.) AGAINST Bacillus Subtilis AND Vibrio Cholerae
DOI:
https://doi.org/10.24233/BIOV.3.1.2017.60Abstract
The purpose of this research to determine the fractions of Chick Weed which has strong antibacterial activity against bacteria test categories of Bacillus subtilis and Vibriocholerae.determine the value of the minimum in hibitory concentration(MIC) of the active fraction antibacterial Chick Weed.This research was carried out in August up to November 2016. The method used in this study were extracted by maceration, fractionation by liquid-liquid fractionation, separation by column chromatography fractions, antibacterial activity test by theKirby-Bauermethod, while the determination of minimum in hibitory concentration by dilution broth,with test bacteria Bacillus subtilis and Vibriocholerae.The data presented in tabular form based on the average value and percent.The results of this study showed that the methanol extract Chick Weed active against test bacteria Bacillus subtilis and Vibrio cholerae. Fractionation which has strong category to standard antibiotics are methanol fraction by fraction column S4.The concentration MIC1000;500;250;125;62.5;31.2515.62; 7.81 ppm. The minimum in hibitory concentration column fractions S4 to test bacteria Vibrio cholerae of 62.5 ppm gives half the antibacterial activity of the antibacterial activity of standard antibiotics streptomycin and penicillin,tetracycline while giving a quarter activity. It can be concluded that the active fraction of methanol extractisa methanol fraction by fraction column S4 to test bacteria Vibrio cholerae.
Keywords: Chick Weed, Minimum Inhibitory Concentration (MIC), active compound, Bacillus subtilis, Vibriocholera.
Last Year PDF Downloads
References
C.W. Bacon, J.F. White, Microbial Endophytes, Marcel Dekker, New York, 2000, p.487.
G.A. Strobel, Biotechnol. 22/4 (2002) 315.
T. Thongchai, L. Chunhua, S. Yuemao, J. Microbiol. 151 (2005) 1691.
G. Strobel, B. Daisy, Microbiol. Mol. Biol. Rev.
/4 (2005) 491.
G.S. Joseph, G.K. Jayaprakasha, A.T. Selvi, B.S. Jena, K.K. Sakariah, Int. J. Food Microbiol. 101 (2005) 153.
N. Nilar, L.H.D. Nguyen, G. Venkatraman, K.Y. Sim, L.J. Harrison, Phytochemistry 66 (2005) 1718.
A.M. Lannang, J. Komguem, F.N. Ngninzeko, J.G. Tangmouo, D. Lontsi, A. Ajaz, M.I. Choudhary, R. Ranjit, K.P. Devkota, B.L. Sondengam, Phytochemistry 66 (2005) 2351.
PDF Downloads: 121
Published
How to Cite
Write scientific names with Italic fonts:
Issue
Section
Copyright (c) 2017 Ratih Anggara, - Munawar, - Elfita
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
d. Authors hold the copyright and retain publishing right of articles without restrictions.
.gif)
