SYNERGISM BETWEEN RHIZOSPHERE BACTERIA ISOLATES FROM Scleria sp., Clidemia sp., AND Panicum sp. TO INCREASE THE EFFECTIVENESS OF MIXED CULTURES IN HYDROCARBON BIODEGRADATION
DOI:
https://doi.org/10.24233/biov.7.2.2021.231Keywords:
hydrocarbon , synergism , rhizosphereAbstract
The purpose of this research is to obtain hydrocarbon degrading bacteria that work synergistically in a consortium. Consortium microorganisms is mixture of microbial populations in the form of communities that have mutualistic relationships and doesn’t inhibition the growth of other microbes. In this study, isolates were obtained from the rhizosphere of soil contaminated with petroleum. The isolates obtained were tested for synergism to determine the relationship between bacterial isolates. Synergism testing was carried out using the spread plate method on agar media. The results of this study showed that isolate number one showed antagonistic properties to other bacterial isolates by forming a clear zone around the disc paper. A total of eight bacterial isolates showed the greatest percentage of synergism, namely ≥ 80% so that the eight rhizosphere bacterial isolates could be used as materials for mixed culture.
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References
Susanti, W.I and Trinanda R, “Potensi Bakteri Asal Tanah Rhizosfer, Sedimen Tanah, dan Pupuk Kandang Sapi Untuk Biodegradasi Minyak Berat dan Oli Bekas” Jurnal Tanah dan Iklim, Vol. 41, No. 1, PP. 37-44, 2017
Khairani, K., Aini, F., & Riany, H, “Karakterisasi dan Identifikasi Bakteri Rhizosfer Tanaman Sawit Jambi”. Journal Biology Al Kauniyah, Vol. 12, No. 2, PP. 198-206, 2019
Kuhad, R.C. and Gupta, R.. 2009. Biological remediation of petroleum contaminants. In Singh, A., R.C. Kuhad and O.P. Ward (Eds.). Advances in Applied Bioremediation. Springer-Verlag, Berlin. 365pp. Soil Biology. 17(9). 173-198. DOI 10.1007/978-540-89621-0_9,
Holifah, S., Supartono, Harjono, “Analisis Penambahan Kotoran Kambing dan Kuda pada Proses Bioremediasi Oil Sludge di Pertambangan desa Wonocolo” Indonesian Journal of Chemical Science. Volume 7 No. 1, PP. 35-42, 2018
Sanchez, O. 2006. A Consortium of Bacteria to Degrade Petrol. Department de Genetica de Microbiologia. Universitat Autonoma de Barcelona
Rifai, M.R. Widowati, H., Sutanto, A, “ Sinergisme dan Antagonisme Beberapa Jenis Isolat Bakteri yang Di konsorsiumkan” Journal of Science and Biology Education, Vol 1 , No 1, PP. 21-26, 2020
Siahaan, M.S., Hutapea, M., & Hasibuan, “Penentuan kondisi optimum suhu dan waktu karbonasi pada pembuatan arang dari sekam padi”. Jurnal Teknik Kimia USU, Vol. 2 No. 1, PP. 26-30, 2013
Purwaningsih, 2004. Pengujian mikroba sebagai pupuk hayati terhadap pertumbuhan tanaman. (Tesis). Institut Pertanian Bogor
Silitonga, D. M., Priyani, N., dan Nurwahyuni, I., “Isolasi dan uji potensi isolat bakteri pelarut fosfat dan bakteri penghasil hormon IAA (indole acetic acid) terhadap pertumbuhan kedelai (Glycine max L.) pada tanah kuning” Jurnal USU, Vol 1, No 2, PP 35-41, 2013
Yudono, B., 2011. Sinergi bakteri tanah dan tanaman pada proses bioremediasi tanah terkontaminasi minyak bumi. (Doctoral Dissertation.). Program Pascasarjana Universitas Sriwijaya
Hartanti, D.A, “Isolasi dan Uji Sinergisme Bakteri Endofit Tanaman Padi (Oryza sativa L.) Untuk Konsorsium Biofertilizer”, Jurnal Agroradix. Vol. 3, No. 2, PP 23-30, 2020
Arora, N.K. 2015. Plant microbes symbiosis: Applied facets. Springer, India
Aditiawati, P., Pikoli, M.R., dan Indriani, D., 2001. Isolasi bertahap bakteri pendegradasi minyak bumi dari sumur bangko. IATMI. Proceeding Simposium Nasional, 59(3): 1-8.
Y. Deng, dan S. Y. Wang, “Synergistic growth in bacteria depends on substrate complexity” Journal Microbiol. Vol 54, No. 1, PP 23-30, 2016
Nugroho, A. 2006. Biodegradasi sludge minyak bumi dalam skala mikrokosmos : simulasi sederhana sebagai kajian awal bioremediasi land treatment. Makara Teknologi, 10(2): 82-89
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