PRIMER OPTIMIZATION AS GENETIC MARKER PRIMER BASED ON CYTOCHROME B GENE ON SUMATRAN ELEPHANT (Elephas maximus sumatranus) NON-INVASIVE SAMPLES

Authors

  • Eko Agus Srihanto Lampung Disease Investigation Center
  • Elly Lestari Rustiati Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung
  • Priyambodo Biology Department
  • Dian Neli Pratiwi Magister of Environmental Sciences, University of Lampung
  • Alvin Wiwiet Susanto Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung
  • Diah Esti Anggraini Prof. Dr. Ir. Rubini Atmawidjaja Elephant Hospital, Way Kambas National Park
  • Enny Saswiyanti Lampung Disease Investigation Center

Article Metrics

This article Abstract has been read: 32 times
PDF Downloads: 24 times
Total Galley Downloads: 24 times
Total Views: 56 times

DOI:

https://doi.org/10.24233/biov.8.1.2022.216

Keywords:

Sumatran Elephant , Way Kambas National Park , Primer Optimization , Genetic Marker , Cytochrome B Gene

Abstract

Sumatran elephant is declared as critically endangered by IUCN since 2011. In supporting conservation efforts on sumatran elephant in its natural habitat, molecular data collection of their population needs to be done. Genetic source material is mostly obtained in its natural habitat is its dung/feces. Study on cytochrome B gene as genetic marker from sumatran elephant fecal samples can be done to get additional genetic data as a form of conservation effort. Primer optimization as genetic marker based on cytochrome B gene has been carried out as an early study on sumatran elephant genetics.  Primer optimization program based on cytochrome B genetic marker from sumatran elephant fecal samples was under Higher Education Applied Research funded by The Ministry of Research, Technology, and Higher Education. DNA was isolated from fecal samples of captive sumatran elephants in Elephant Training Center, Way Kambas National Park. Primer optimization was done via amplification by increasing certain temperature. Specificity test was done to ensure that the primer only recognized sumatran elephant DNA. The best result was obtained with the annealing temperature of 52oC

Last Year PDF Downloads

Download data is not yet available.

References

Gopala, A., Hadian, O., Sunarto, ., Sitompul, A., Williams, A., Leimgruber, P., Chambliss, S.E. dan Gunaryadi, D. 2011. Elephas maximus ssp. sumatrensis. The IUCN Red List of Threatened Species 2011.

Kementerian Lingkungan Hidup dan Kehutanan. 2020. Rencana Tindakan Mendesak Penyelamatan Gajah Sumatera (Elephas maximus sumatranus) 2020-2023. Kementerian Lingkungan Hidup dan Kehutanan. Jakarta.

Fernando, P., Vidya, TNC., Payne, J., Stuewe, M., Davison, D., Alfred, RJ., Andau, P., Bosi, E., Kilbourn, A., Melnick, DJ. 2003. DNA Analysis Indicater that Asian Elephants are Native to Borneo and are Therefore a High Priority for Conservation. PloS Biology 1 (1):110-115

Sulandari, S. & Zein, MSA. 2012. Mitocondrial DNA Variation of the Sumatran Elephant Populations in Sumatera, Indonesia. BIOTROPIA 19 (2): 92-102.

Avise, J.C. 2004. Molecular Markers, Natural History and Evolution. Sinauer Associates. Sunderland, Massachusetts.

Borisenko, AV., BK. Lim, NV. Ivanova, RH. Hanner & PDN. Hebert. 2008. DNA barcoding in surveys of small mammal communities: afield study in Suriname. Molecular Ecology Resources 8:471479.

Hebert, PDN., A. Cywinska, SL. Ball & JR. deWaard. 2003. Biological identificationsthrough DNA barcodes. Proceedings of the Royal Society of London. Biological Sciences, Series B. 270: 313–322.

Hajibabaei M., DH. Janzen, JM. Burns, W. Hallwachs & PDN. Hebert. 2006. DNA barcodes distinguish species of tropical Lepidoptera. Proceedings National Academy of Sciences. USA. 103(4) 968971.

Ivanova, NV., JR. deWaard, PDN. Herbert. 2006. An inexpensive, automation-friendly protocol for recovering high quality DNA. Molecular Ecology Notes. 6: 998–1002

Rustiati, E.L., Priyambodo, T. Novianasari, D.N. Pratiwi, E.A. Srihanto and L. Angeliya. 2019. Comparative study on DNA Extraction’s qualitative analysis of captive Sumatran elephant in Elephant Training Center, Way Kambas National Park. Journal of Physics: Series 1338 (2019) 012029.

Dieffenbach, C.W., T. M. Lowe, and G. S. Dveksler. 1993. General concepts for PCR primer design. Genome Res. 3: S30-S37.

Abd-Elsalam, K.A. 2003. Bioinformatics tools and guideline for PCR primer design. Afr. J. Biotechnol. 2(5): 91-95.

Cateno-Anolles, G., Bassam, B.J and Gresshoff, P.M. 1991. High resolution DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Biotechnology 9:553-557

OIE Terestrial Manual, 2008, Principles of validation of diagnostic assays for infectious diseases, Chapter 1.1.4

Virnarenata, E., Rustiati, E. L., Priyambodo, P., Srihanto, E. A., & Pratiwi, D. N. 2021. Identification and Characterisation of Coi Gene in Female Sumatran Elephant (Elephas maximus sumatranus) In Elephant Training Centre, Way Kambas National Park. BIOVALENTIA: Biological Research Journal, 7(1): 1-4.

Indriati, W., Yustian, I., & Setiawan, A. 2020. Quantitative and Qualitative Test of The Fecal Sampel From Sumatran Elephant (Elephas maximus sumatranus). BIOVALENTIA: Biological Research Journal, 6(2).

Published

31-01-2022

How to Cite
Write scientific names with Italic fonts:

Srihanto, E. A., Rustiati, E. L., Priyambodo, P., Pratiwi, D. N., Susanto, A. W. ., Anggraini, D. E. ., & Saswiyanti, E. . (2022). PRIMER OPTIMIZATION AS GENETIC MARKER PRIMER BASED ON CYTOCHROME B GENE ON SUMATRAN ELEPHANT (Elephas maximus sumatranus) NON-INVASIVE SAMPLES. BIOVALENTIA: Biological Research Journal, 8(1), 32–35. https://doi.org/10.24233/biov.8.1.2022.216

Issue

Section

Vol 8, No 1 (2022): May 2022

Most read articles by the same author(s)